人類miRNA與乳癌抑制基因之可能性探討

Human microRNAs Target Candidate Breast Tumor Suppressor Genes

林育如(lin yo ju)

指導教授 ： 吳家樂

亞洲大學/資訊學院/生物資訊學系碩士班/碩士(2007年)

摘要

MicroRNA (miRNA)是一群非常短的RNA，大約只有二十二個鹼基長，其最明顯的特徵就是具有一個髮夾的構造(hairpin)。miRNA 在基因轉譯(transcription)成蛋白質過程中扮演著重要角色。同時目前也認為miRNA 在腫瘤發生發展過程中，具有與致癌基因或抑癌基因相似的作用，有些致癌病毒也編碼miRNA 影響宿主細胞，在腫瘤發生中可能具有重要的作用。
本研究主要是對於乳癌的miRNA 標靶基因做確認。我們從三個主要的乳癌microarray 實驗確認預測的標靶基因其基因的表達狀態是被抑制的。結果顯示miRNA 可能抑制和乳癌有關的基因。這些miRNA可能扮演著調控抑制腫瘤基因的角色，我們將再從HPRD 和OMIM 資料庫提供的生物功能和疾病訊息做進一步調查研究。

關鍵字

microRNA ； 乳癌、抑癌基因

並列摘要

MicroRNA (miRNA) is an extremely short chain of RNA, containing approximately twenty-two basepairs and a prominent characteristic of hairpin structure. miRNA also plays an essential role in the process of gene transcription into protein. Meanwhile, it is generally accepted that, during the development of tumor, miRNA functions in a similar way to oncogene or Tumor Suppressor Gene (TSG). Certain viruses able to induce cancer encode miRNA, and accordingly have an impact on host cells and behave as gene regulator.
This research mainly aims at identifying miRNAs targeting regions ,in which their gene expression levels is down-regulated according to three selected primary breast tumor microarray experiments. These results propose that these miRNAs can possibly suppress the expression level of these breast cancer related genes. The possibility of these miRNA genes in the role of the tumor suppressor genes is further nvestigated by retrieving their biological functions and disease nformation from the HPRD and OMIM databases.

並列關鍵字

microRNA ； Breast Cancer , Tumor Suppressor Gene

參考文獻

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