葡萄花青素合成路徑調控因子之文獻探討及酵素動力學參數預測

Literature review and kinetic parameters studies of the grape anthocyanin biosynthesis pathway

潘恩祺(pan ên chi)

指導教授 ： 吳家樂

亞洲大學/資訊學院/生物資訊學系碩士班/碩士(2007年)

摘要

本研究以現有各大生物資訊資料庫所提供的資料與工具，蒐集相關資訊來探討影響葡萄花青素合成的物理、化學因素及調控因子。並且利用序列同源性之關係，歸納及預測參與此生化反應的酵素Km值，提供植物細胞模擬花青素合成進階研究之基礎。 此研究以KEGG 資料庫提供的花青素合成之生化路徑為架構，參考NCBI、BRENDA、SABIO-RK 等資料庫，來建立相關研究資料，呈現具體、系列化的資訊，建立完整表格，可提供實驗室對花青素複雜的代謝途徑更進階之研究，或許可以應用來提高花青素產能之參考。論文中歸納了參與花青素合成的重要酵素，詳述各個階段酵素參與反應的受質與產物，更表列了參與生化反應的物理、化學調控因子，也搜尋相關酵素的調控基因位點、結構基因位點，提供細胞分子層級的基因調控資料，提供進階研究之用。

關鍵字

花青素、類黃酮類、自由基、調控基因、結構基因

並列摘要

This research proposes to study the physical,chemical and genetic factors that could affects the grape (Vitis vinifera) anthocyanin biosynthesis pathway by integrating various kinds of molecular biology database information and through literature reviewing .Furthermore ,the kinetic parameter and upstream regulatory proteins of the anthocyanin are predicted based on the sequence homology principle.
The grape anthocyanin biosynthesis pathway is retrieved from the KEGG database .Other utilized databases such as NCBI,BRENDA and SABIO-RK are useful for the collection of kinetic parameters.
Several physical and chemical factors that could possibly enhanced the anthocyanin production are identified .It is also found that the Myb and Myc protein families could be the upstream regulating proteins for certain enzymes involved in the pathways .
These results could be a useful piece of information for the grape anthocyanin biosynthesis pathway further study.

並列關鍵字

anthocyanins、flavonoids、Free radicals、structural genes、regulatory genes

參考文獻

邱輝龍、范明仁。 1998. 花青素與花色之表現。 中國園藝44(2):102-115。

施怡如、鄭統隆、曾東海、王強生。2004. 有色水稻中花青素的種類及其功能探討。 中華農業研究53:221-228。

陳惠英、顏國欽 。1998. 自由基、抗氧化防禦與人體健康 Nutr. Sci.J. 23(1):105-121.

Arakawa, O. 1991. Effect of temperature on anthocyanin accumulation in apple fruit as affected by cultivar, stage of fruit ripening and bagging. J. Hort. Sci. 66：763-768.

Harborne J, Williams C (2000) Advances in flavonoid research since 1992. Phytochemistry 55: 481–504