SARS_CoV spike蛋白質 match CD4 預測

The prediction of SARS_CoV spike protein binding CD4+T cell

孫瑞強(sun ray chung)

指導教授 ： 李桂仁

亞洲大學/資訊學院/生物資訊學系碩士班/碩士(2005年)

摘要

為了獲得the S protein of SARS_CoV and CD4之間的ligand-receptor binding的資料,確定兩者之間可能作用的胺基酸的範圍,和binding sites的有關資料,並希望這些資料能對製造 抗SARS 的要或者疫苗有所幫助。
本預測用各種生物資訊的工具,如找出consensus domain,homology search以及用AutoDocking sofewar來預測CD4 和S protein of SARS_CoV的interacting domains 和binding sites 又用各種生物資訊的工具預測出SARS_CoV S protein t對CD4可能的binding部分為 SARS_CoV S 蛋白質的 T1059—C1064結合到 CD4 的A126-F203 domain,對於這一結果,我們也以生物資訊的工具將它製圖出來。
CD4可能結合SARS_CoV S的蛋白質,這可能也和SARS的感染有關,這份報告可能供給我們一些設計醫療SARS的藥或疫苗有所幫助。

關鍵字

SARS_CoV spike蛋白質 match CD4 預測

並列摘要

To obtain the information of ligand-receptor binding between the S protein of SARS_CoV and CD4, identify the possible interacting domains or motifies related to binding sites, and provide clues for studying the functions of SARS proteins and designing anti-SARS drugs and vaccines.
We used the homology search, and multi-sequence alignment were used to predict CD4 related interacting domains and binding sites in the S protein of SARS_CoV. Molecular modeling and using AutoDock methods were employed to address the interaction feature between CD4 and S protein of SARS_CoV in validating the bioinformatics predictions.
Possible binding sites in the SARS_CoV S protein to CD4 have been Mapped out by using bioinformatics analysis tools. The binding for one protein-protein interaction pair (T1059—C1064 motif of the SARS_CoV S protein to A126-F203 domain of CD4) has been simulated by molecular modeling and AutoDock software.
CD4 may be a possible receptor of the SARS_CoV S protein, which may be associated with the SARS infection.This result may give us some methods to design anti-SARS drugs and vaccines.

並列關鍵字

The prediction of SARS_CoV spike protein binding CD4+T cell

參考文獻

[4] Dr. Richard Hunt.Microbiology and Immunology On-Line.UNIVERSITY OF SOUTH CAROLINA.SCHOOL OF MEDICINE. Coronaviruses.htm. Microbiology & Immunology:BS3035:Coronaviruses Updated: May 1,2003.

[6] Kathryn V.Holmes,”SARS-Associated Coronavirus,” N ENGL J MED, 2003.Vol.

[7] John Ziebuhr,1 Eric J. Snijder2 and Alexander E. Gorbalenya3,” Virus-encoded proteinases and proteolytic processing in the Nidovirales,”J Gen Virol.2000.81(Pt 4):853-79.

[8] Germain R N, Stefanova I,”The dynamics of T cell receptor signaling: complex orchestration and the key roles of tempo and cooperation,”Annu Rev Immunol. 1999.17:467-522.

[9] Porcelli SA, Modlin RL,”The CD 1 system:antigen-presenting molecules for T cell recognition of lipids and glycolipids,” Annu Rev Immunol .1999;17:297-329.