食道癌細分型相關之mRNAs及microRNAs

Esophageal cancer subtype-related mRNAs and micoRNAs

陳桂冠(Kuei-Kuan Chen)

指導教授 ： 吳家樂

亞洲大學/資訊學院/生物資訊學系碩士班/碩士(2008年)

摘要

從病理學的角度而言，食道癌可細分為鱗狀上皮細胞癌（squamous sell carcinoma）及腺癌（adenocarcinoma）兩種，另外巴瑞特氏食道症（Barrett's Esophagus）被視為導致食道腺癌的癌前病變。
目前有研究顯示microRNAs可能為調控細胞癌化及其他疾病的重要因子，因此找出microRNAs標靶癌症基因，將能提供診斷與治療癌症的新方法。本研究主要是藉由分析食道癌病人中正常組織和癌症組織的微陣列基因表現數據，經由T-test檢定篩選出有差異性表現的基因，再預測這些基因與人類的microRNAs標靶的可能性，標靶到的miRNA即有可能扮演差異表達基因的角色。
此外，本研究也對食道癌亞型的相關基因作進一步分析，篩選出特異性基因，期能作為預測亞型的生物標記。

關鍵字

微型核糖核酸 ； 食道癌 ； 鱗狀上皮細胞癌 ； 腺癌 ； 巴瑞特氏食道症

並列摘要

In terms of pathology, esophageal cancer can subdivide into squamous sell carcinoma (SCC) and adenocarcinoma (ADC). In addition, Barrett's Esophagus (BE) is considered as the pathological change before the induction of esophageal adenocarcinoma.
At present some researches demonstrate that microRNAs could possibly regulating the development of cancers and other diseases. Therefore, discovering microRNAs targeting cancer genes will allow one to provide a new method to diagnose and treat cancer.
In this thesis, we use t-test to identify sets of differential expressed genes for esophageal cancer. The putative miRNA targeting mRNAs are obtained by using miRanda. Certain miRNA could possibly bind with mRNAs, and this suggested that miRNAs could possible play the role of regulating differential expression genes. Furthermore, we also identified sets of esophageal cancer subtype specific genes, these genes could sever as a biomarker for subtype classification.

並列關鍵字

microRNA ； esophageal cancer ； squamous sell carcinoma ； adenocarcinoma ； Barrett's Esophagus

參考文獻

[1]Gupta P.K.(2006) RNA interference–gene silencing by double-stranded RNA:The 2006 Nobel Prize for Physiology or Medicine. Current Science 91(11), pp.1443-46.

[2]Szymański M, Erdmann VA, Barciszewski J.(2003)Noncoding regulatory RNAs database. Nucleic Acids Research 31(1), pp.429–31.

[3]Eddy S.R. (2001) Noncoding RNA genes and the modern RNA world. Nature 2(12), pp.919-29.

[4]He L., Hannon G. J. (2004). MicroRNAs: small RNAs with a big role in gene regulation. Nat. Rev. Genetics 5(7), pp.522-31.

[6]Reinhart B.J., Slack F.J., Basson M., Pasquinelli A.E., Bettinger J.C., Rougvie A.E., Horvitz H.R., Ruvkun G., (2000).The 21 nucleotide let-7 RNA regulates C. elegans developmental timing, Nature 403(6772), pp.901-06.