麻薏對脂多醣活化巨噬細胞株發炎反應之影響

Effects of Corchorus capsularis on inflammation by LPS-activated macrophage cell line

朱曼萍(MAN-PING CHU)

指導教授 ： 趙哲毅

亞洲大學/健康學院/保健營養生技學系碩士班/碩士(2009年)

摘要

麻薏 (Corchorus capsularis) 為黃麻之嫩葉，本研究探討麻薏不同部位其萃取物的抗氧化力和調控發炎反應之能力。實驗中利用Trolox 當量濃度來換算其抗氧化之效果，結果顯示麻薏碎葉乙醇萃取物 (CGLEE) 經細胞處理後，經換算其Trolox濃度為4.12 μM，表示該樣品有較佳的抗氧化功效。
在短暫轉染實驗中，則以麻薏葉脈熱水萃取物 (CVHWE) 91.6％活化PPARγ效果最明顯。在細胞存活率方面，麻薏各萃取物在100 μg/mL之濃度下與巨噬細胞共同培養後其細胞存活率均無顯著差異(p＞0.05)。抑制經由脂多醣誘導巨噬細胞RAW264.7細胞生成NO2之表現的能力，麻薏完整葉乙醇萃取物 (CCLEE)、麻薏含葉脈熱水萃取物 (CLCVHWE) 均能顯著抑制LPS所誘導RAW264.7細胞生成NO2之作用，而麻薏葉去葉脈熱水萃取物 (CLRVHWE) 則無效果。
在PGE2生成結果中，發現麻薏完整葉乙醇萃取物 (CCLEE) 在減少PGE2之生成量效果最佳，且有統計上之顯著差異；而在麻薏熱水萃取物部份則無差異。對於LPS誘導RAW264.7巨噬細胞株COX-2蛋白質之表現情形，以麻薏碎葉乙醇萃取物 (CGLEE) 降低COX-2蛋白表現效果最為顯著 (p＜0.05)，在麻薏水洗葉部分雖然抑制COX-2蛋白質之表現，但其PGE2之生成量與控制組相當。
對於NF-κB蛋白質表現上，與控制組相比麻薏莖甲醇萃取物 (CSME)、麻薏含葉脈熱水萃取物 (CLCVHWE) 有顯著抑制NF-κB p65蛋白質之表現。綜合上述結果，以麻薏碎葉乙醇萃取物 (CGLEE) 抑制NO2、PGE2生成及抑制COX-2蛋白表現有顯著功效，且具有降低NF-κB蛋白質表現之趨勢。

關鍵字

麻薏、抗發炎、過氧化體增殖劑活化受器-γ、環氧化酶-2、前列腺素E2

並列摘要

This study was discussed the different parts of Corchorus capsularis, and their antioxidant activity and the ability on the inflammatory effects by LPS-activated macrophage cell line. In the antioxidant activity, CGLEE had the best suppression. In transient transfection assay showed that the CVHWE activated PPARγ significantly. The MTT assay results showed that Corchorus capsularis extracts treatments did not significantly influence the cell viability. However, the Corchorus capsularis leaf reject vein hot water extract (CLRVHWE) had no effect. The CCLEE could inhibited the PGE2 synthesis, and Corchorus capsularis hot water extract had no significantly difference. In addition, suppression on LPS-induced COX-2 protein expression of RAW264.7 showed that CGLEE was the best. The CLCVHWE and CLRVHWE inhibited COX-2 protein expression, but PGE2 had no different with vehicle. In the NF-κB p65 protein expression, the CSME and CLCVHWE had significant ability inhibition. In conclusion, the effects of CGLEE could suppressed NO2, PGE2 and COX-2 protein expression and decreased NF-κB p65 protein expression.

並列關鍵字

Corchorus capsularis，anti-inflammation，PPARγ，COX-2，PGE2

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