舞菇萃取物抗氧化活性之研究

Antioxidant properties of the extracts from Grifola frondosa

謝歷輝(Li Hui Hsieh)

指導教授 ： 蔡政芳

亞洲大學/健康學院/生物科技學系碩士班/碩士(2010年)

摘要

舞菇(又稱Maitake)可利用的子實體和菌絲體中過去曾被指出具有抗氧化的成分。本研究將T1舞菇和T2舞菇的子實體粉末以乙醇、冷水和熱水方式萃取來用於抗氧化活性的測定。抗氧化活性的關連性將以還原力、亞鐵離子螯合能力、DPPH自由基清除能力及超氧陰離子清除能力之EC50來評估。在濃度1 mg/mL時，T1和T2舞菇的冷水萃取物具有較高之還原力，其吸光值為1.02和0.50。T1和T2舞菇的亞鐵離子螯合能力方面冷水萃取物具有較高的螯合能力，其次是乙醇和熱水萃取物。T1和T2舞菇的DPPH自由基清除能力方面，清除力的順序為: 乙醇萃取物 > 熱水萃取物 > 冷水萃取物。T1和T2舞菇的超氧陰離子清除能力方面，熱水萃取物具有較高之清除力。總酚類化合物、類黃酮化合物、抗壞血酸、α-生育醇及β-胡蘿蔔素為T1和T2舞菇萃取物的抗氧化成分並做含量測定。以EC50結果評估，T1和T2舞菇的3種萃取物具有不錯的抗氧化活性。

關鍵字

舞菇 ； 抗氧化 ； 成分分析

並列摘要

Grifola frondosa (Maitake) is available in the form of fruit body and mycelium has been reported to contain antioxidant omponents. T1 maitake and T2 maitake ethanolic, cold water and hot water extracts were prepared form fruit body and their antioxidant properties studied. Correlations of contents of antioxidant components with EC50 values of reducing power, chelating ability on ferrous ions, scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) and scavenging ability on superoxide anion were established. At 1 mg/mL, T1 and T2 cold water extract showed a high reducing power of absorbance were 1.02 and 0.50. Chelating abilities on ferrous ions of T1 and T2, cold water extracts were higher than ethanolic and hot water extracts. With regard to the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), T1 and T2 extracts were effective in the order: ethanolic > hot water > cold water extracts. With regard to the scavenging ability on superoxide anion, T1 and T2 hot water extract showed a high scavenging ability.Total phenols, flavonoid, ascorbic acid, α-tocopherol and β-carotene were the antioxidant components found in T1 and T2 extracts. Based on EC50 values, three extracts from T1 and T2 were good in antioxidant properties.

並列關鍵字

Grifola frondosa ； antioxidant ； antioxidant components

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