PURPOSE: The purposes of the study were to investigate: (1) the effects of one week swimming training in cold (17°C) and normal (24°C) water conditions (CWT & NWT) on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). (2) the effects of cold water swimming on SOD, CAT, GSH-Px and malondialdehyde (MDA) at 105% critical velocity (CV) intensity swimming test between CWT and NWT. METHODS: Fourteen highly trained swimmers with average age of 17.36 ±1.86 years, height of 171.71 ±7.51 cm and body weight of 66.64 ±8.23 kg, were randomly divided into CWT and NWT groups. Venous blood samples were taken before and after training. Measurements of 50m, 100m, 200m and 400m front crawl were performed to determine CV during four days; the other regular training programs were performed. The seventh day, both groups were tested with 105% CV swimming intensity, and blood samples were taken before and after the test to determine CAT, SOD, GSH-Px and MDA responses. Assay kits analyzed the concentration of CAT, SOD, GSH-Px and MDA. Mixed design three-way ANOVA was used to analyze the difference among two groups, the 11 level was set at 0.05. RESULTS: There were no interaction between cold water stimulate and all variances in both purposes, advance simple main effect indicated the rest SOD, and MDA were statistically significant difference in CWT (F=16.95, 6.46, p<.05) and GSH-Px and MDA statistically significant difference in NWT (F=8.10, 14.22, p<.05) after one week swimming training. In the 105% CV swimming intensity test, GSH-Px activity was significantly increased in CWT (F=43.30, p<.05), and SOD, GSH-Px and MDA were significantly increased in NWT (F=11.11, 35.29, 21.05, p<.05). CONCLUSION: One week of cold water stimulation did not improve the rest and 105% CV swimming intensity CAT, SOD and GSH-Px levels. However, one week swimming training and after 105% CV swimming intensity test increased some antioxidants activity and lipid peroxide in both groups. It is suggested that training effect is better than cold-water stimulation to improve antioxidant activity.
PURPOSE: The purposes of the study were to investigate: (1) the effects of one week swimming training in cold (17°C) and normal (24°C) water conditions (CWT & NWT) on superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). (2) the effects of cold water swimming on SOD, CAT, GSH-Px and malondialdehyde (MDA) at 105% critical velocity (CV) intensity swimming test between CWT and NWT. METHODS: Fourteen highly trained swimmers with average age of 17.36 ±1.86 years, height of 171.71 ±7.51 cm and body weight of 66.64 ±8.23 kg, were randomly divided into CWT and NWT groups. Venous blood samples were taken before and after training. Measurements of 50m, 100m, 200m and 400m front crawl were performed to determine CV during four days; the other regular training programs were performed. The seventh day, both groups were tested with 105% CV swimming intensity, and blood samples were taken before and after the test to determine CAT, SOD, GSH-Px and MDA responses. Assay kits analyzed the concentration of CAT, SOD, GSH-Px and MDA. Mixed design three-way ANOVA was used to analyze the difference among two groups, the 11 level was set at 0.05. RESULTS: There were no interaction between cold water stimulate and all variances in both purposes, advance simple main effect indicated the rest SOD, and MDA were statistically significant difference in CWT (F=16.95, 6.46, p<.05) and GSH-Px and MDA statistically significant difference in NWT (F=8.10, 14.22, p<.05) after one week swimming training. In the 105% CV swimming intensity test, GSH-Px activity was significantly increased in CWT (F=43.30, p<.05), and SOD, GSH-Px and MDA were significantly increased in NWT (F=11.11, 35.29, 21.05, p<.05). CONCLUSION: One week of cold water stimulation did not improve the rest and 105% CV swimming intensity CAT, SOD and GSH-Px levels. However, one week swimming training and after 105% CV swimming intensity test increased some antioxidants activity and lipid peroxide in both groups. It is suggested that training effect is better than cold-water stimulation to improve antioxidant activity.