- 期刊
移植骨生成細胞預防年齡增長後骨質流失效果及機轉的骨組織形態學研究
A Bone Histomorphometric Study of Preventive Effects and Mechanism of Transplanted Osteogenic Cells on Age-related Osteopenia
摘要
隨著年齡的增長，骨量（或骨質密度）的減少可造成骨質流失或增加骨折發生的機會。發生骨折後，可能造成局部功能喪失或甚至有生命之危險。年齡增長後，除了骨質吸收顯著增加外，骨生成也有明顯降低的情形。年齡增長後骨生成明顯的減少導致骨量減少是因為骨髓細胞中之造骨原始細胞增生和分化和造骨細胞活性顯著減少所造成。至今，尚未有任何文獻報導骨生成細胞對年齡增長後骨質流失或疏鬆預防效果及可能機轉之研究。因此，本研究旨在探討移植骨生成細胞防治年齡增長後骨質明顯流失效果及機轉研究。方法：自17月大白鼠取得骨髓細胞並進一步純化、培養和誘導成為分泌BMP之骨生成細胞後植入並分別利用血清骨生化參數和骨組織形態學靜態和動態參數評估法進行結果分析。實驗結果，發現植入骨生成細胞1個月後，單位面積造骨細胞數目（OB/mm²）、骨生成速率（1.87±0.03）和礦物質沈積速率（4.11±0.23）均有顯著增加趨勢。而血清中抗酒石酸鹽酸性磷酸酶（TRAP）濃度，單位面積破骨細胞數目（OC/ mm²）和破骨細胞骨吸收面積（RS）兩者均無顯著變化（影響）。因此，本研究結果顯示移植骨髓骨生成細胞有效預防年齡增長後骨質流失和增加骨生成。
並列摘要
With aging, the decrease in bone mass (or bone mineral density) could cause osteoporosis or increase the occurrence of fracture. A fracture could result in loss of function or even death. Therefore, investigations on keeping bone tissue and cells functionally and prevention of damage of bone structure are imperative. With aging, dramatic bone loss and a significant decrease in bone formation are noted. Previous studies reported that there are significant decrease in proliferation and differentiation of osteoprogenitor cell and osteoblastic function with aging. In addition, accumulated data revealed that BMP-2 has many therapeutic uses in orthopedics for repair of nonunions, large bone defect, promotion of spinal fusion, stabilization of prosthetic devices, and in the treatment of fractures in osteoporotic patients. However, up to now, no data available regarding therapeutic potential of transplantation of BMP-2-producing osteogenic cells on osteopenia with aging. Therefore, the purposes of this study was to investigate the effects and mechanism of transplantation of BMP-2 producing osteogenic cells on osteopenia with aging. There were significant increases in number of osteoblasts, bon formation rate and mineral appositional rate while no obvious differences in serum tartrate-resistant acid phosphatase level, number of osteoclasts, resorption surface one month after transplantation. It is concluded that transplantation of bone marrow osteogenic cells could effectively prevent age-related osteopenia.