Parathyroid Hormone Enhances Osteoblast Differentiation: An in Vitro Study

副甲狀腺素增進造骨細胞分化的體外研究

史中(Chung Shih)

《中華民國口腔顎面外科學會雜誌》 15卷2期 (2004/09) Pp. 85-91

https://doi.org/10.7104/CJOMS.200409.0085

摘要

With aging, the decrease in bone mass (or bone mineral density) could cause osteoporosis or increase the occurrence of fracture. It ultimately involves all the soft tissues in the injured area as well as the bone, resulting in loss of function or even death. Current evidences showed that there is an increase in bone mass in both osteoporotic patients and animals treated with low dose of parathyroid hormone (PTH). In addition, PTH could increase cortical bone volume and prevent postovariectomized osteopenia. However, the precise effect of PTH on bone cell differentiation is still unclear. The purpose of this study was to investigate the precise effect of low dose PTH on osteoblast differentiation. Cell culture system, (superscript 3)H-thymidine incorporation assay, alkaline phosphatase activity assay, type I collagen synthesis assay, static and dynamic bone histomorphometry and light microscopy were performed on MC3T3-E1 cells. Low dose of hPTH (human PTH) significantly increased osteoprogenitor cell proliferation (p＜0.01 and 0.005) and osteoblastic alkaline phosphatase activity (p＜0.05 and 0.01) while no evidence of significant effect on collagen synthesis. In conclusion, low dose of PTH effectively enhances osteoblast differentiation.

關鍵字

骨質密度 ； 造骨細胞 ； 骨質疏鬆 ； 副甲狀腺素

並列摘要

With aging, the decrease in bone mass (or bone mineral density) could cause osteoporosis or increase the occurrence of fracture. It ultimately involves all the soft tissues in the injured area as well as the bone, resulting in loss of function or even death. Current evidences showed that there is an increase in bone mass in both osteoporotic patients and animals treated with low dose of parathyroid hormone (PTH). In addition, PTH could increase cortical bone volume and prevent postovariectomized osteopenia. However, the precise effect of PTH on bone cell differentiation is still unclear. The purpose of this study was to investigate the precise effect of low dose PTH on osteoblast differentiation. Cell culture system, (superscript 3)H-thymidine incorporation assay, alkaline phosphatase activity assay, type I collagen synthesis assay, static and dynamic bone histomorphometry and light microscopy were performed on MC3T3-E1 cells. Low dose of hPTH (human PTH) significantly increased osteoprogenitor cell proliferation (p＜0.01 and 0.005) and osteoblastic alkaline phosphatase activity (p＜0.05 and 0.01) while no evidence of significant effect on collagen synthesis. In conclusion, low dose of PTH effectively enhances osteoblast differentiation.

並列關鍵字

Bone mineral density ； Osteoblast ； Osteoporosis ； Parathyroid hormone