知母萃取物之抗氧化活性試驗

Studies on the Antoxidant Activity of Anemarrhenae asphodeloides Bge. Extracts

陳玉芳(Yu-Fang Chen)

《傳統醫學雜誌》 24卷1期 (2013/10) Pp. 13-24

摘要

在本實驗中，我們以中藥知母作為抗氧化活性試驗篩選，並比較不同萃取溶劑下，抗氧化活性的差異，抗氧化活性試驗的評估方法包括清除α,α-Diphenyl-β-picrylhydrazyl（DPPH）自由基、螯合亞鐵能力（Chelating activity on ferrous ion）與還原能力（Reducing power test）測定，並配合總多酌類含量（Total phenolic compound content）測定。經實驗結果顯示知母酒精萃取物具有最明顯的清除DPPH自由基能力，其IC50值為0.11 + 0.01mg/ml，另外，以水萃取物其有最高的總多酚含量，其值為18.56mg GA/g。因此，知母酒精萃取物具有優異的抗氧化能力，具有在保健食品和化妝品工業上應用與開發之潛力。

關鍵字

知母 ； 抗氧化活性 ； DPPH自由基 ； 螯合亞鐵離子 ； 還原能力

並列摘要

This study was aimed to evaluate the antioxidant activities of three different extracts from Anemarrhenae asphodeloides Bge. The antioxidant activities including the DPPH radical scavenging effect, Chelating activity on ferrous ion, Reducing power test and Total phenolic compound content. We found that the Anemarrhenae asphodeloides antioxidant activities of EtOH extract had significantly the DPPH radical scavenging effect and The Chelating activity on ferrous ion. The IC50 number were 0.11±0.01mg/ml and 0.09±0.01mg/ml, respectively. The results presented that EtOH extract from Anemarrhenae asphodeloides had antioxidant activity. Thus, EtOH extract from Anemarrhenae asphodeloides possess a broad application and exploitation for cosmetic industry.

並列關鍵字

Anemarrhenae asphodeloides ； Antioxidant activity ； DPPH radical ； Chelating activity on ferrous ion ； Reducing power test ； Total phenolic compound content