Therapeutic aphresis removes abnormal composition which based on extracorporal circulation technique to separate blood component. The nursing skill of performing double filtration plasmapheresis procedure (DFPP) is introduced in this article. Because of the unique equipment, the operator is mostly unfamiliar with this skill. If there is error piping installation or omitting steps, it would jeopardize patients’ safety. The principle of technique and operational experiences were elaborated in detail. This article is expected to provide the clinical guidance and reference to improve the quality of patient care.