Guided tissue regeneration and guided bone regeneration have been used in periodontics and implant dentistry for more than fifteen years. Generally, these procedures can get favorable results if there is no early membrane exposure. Many studies have shown that the result is unpredictable or even failure if membrane has early exposure or infected. Therefore, wound primary closure is absolute influential in the success of regeneration surgery. The important factors to get primary wound closure in regeneration surgery are the right choice and manipulation of suture materials and methods. The purpose of this paper was to present clinical cases to demonstrate the application of various suture methods including mattress suture and modified mattress suture on tissue regeneration surgery.