天麻的抗癲癇作用與Interleukin-1β、Tumor Necrosis Factor-α及一氧化氮關係

Relationship between the Anticonvulsion Effect of Gastrodia elata and Interleukin-1β，Tumor Necrosis Factor-α and Nitric Oxide

黃昱翰(Yu-Han Huang) ； 張永明(Yong-Ming Chang) ； 沈建忠(Jiann-Jong Shen) ； 謝慶良(Ching-Liang Hsieh)

《Mid-Taiwan Journal of Medicine》 10卷s期 (2005/06) Pp. s1-s8

https://doi.org/10.6558/MTJM.2005.10(s).1

摘要

目的　我們先前的研究已知天麻能減少kainic acid(KA)誘發大鼠的癲癇發作和抑制microglia的活化，因此本研究目的是探討天麻的抗癲癇作用與interleukin-1β(IL-1β)、tumor necrosis factor-α(TEN-α)，及一氧化氮(nitric oxide：NO)的關係。
方法　使用KA 12mg於Sprague-Dawley(SD)大鼠腹腔注射誘發癲癇發作，觀察天麻1.0g/kg、0.5g/kg，以及Valproic acid(VA)250mg/kg前治療四天對濕狗甩頭(wer dogshakes)，血液中IL-1β，血液中的IL-1β、TNF-α和NO的濃度，以及前額葉與海馬區IL-1、α、TNF-α濃度的影響。
結果　天麻1.0 g/kg和VA 250mg/kg能減少KA誘發濕名狗甩頭次數和周邊血液IL-1β和一氧化氮的濃度，以及鼠腦前額葉IL-1β、TNF-α和海馬區TNF-α的濃度。
結論　天麻的抗癲癇作用抑制細胞因子IL-1β、TNF-α的濃度。

關鍵字

天麻 ； interleukin-1β ； kainic acid ； 一氧化氮 ； tumor necrosis factor-α ； 濕狗甩頭

並列摘要

Purpose Our previous studies have shown that Gastrodia elata (GE) reduces kainic acid (KA)-induced epileptic seizures and inhibits activation of microglia in rates. The present study investigated the relationship between the anticonvulsion effects of GE and interleukin-1β (IL-1β), tumor necrosis factor-α (TNE-α), as well as nitric oxide (NO).
Methods Rats were administered KA (12mg/kg) to induce epileptic seizure. After oral administration of GE 1.0g/kg, 0.5g/kg and valproic acid (VA) 250mg/kg for 4 days prior to K injection of the number of KA-induced we dog shakes was calculated, and the changes in IL-1β, TNF-α and NO levels in the peripheral blood, and IL-1β and TNF-α levels in the frontal cortex and hippocampus regions of the rat brain were measured.
Results Both pretreatment of GE 1.0g/kg and VA 250mg/kg reduced the number of wet dog shakes. GE also reduced the levels of IL-1β and NO in the peripheral blood, and the levels of IL-1β and TNF-α in the frontal cortex, and TNF-α in the hippocampus.
Conclusions GE inhibits the generation of cytokine IL-1β, TNF-α, and NO in rats with KA-induced seizures.

並列關鍵字

gastrodia elata ； interleukin-1β ； kainic acid ； nrtric oxide ； tumor necorsis factor-α ； wet dog shakes