The purpose of this research was to build the genetics problem solving processes model and based on it to develop and validate Genetics Problem Solving Test (GPST) as the measurement instrument of 10th grade students. The genetics problem solving processes model which was build by integrating some other models included five stages: 1.understand problem, 2.explore problem, 3.select problem solving strategies, 4.excute problem solving, and 5.review and evaluate. According to the testing result of 477 10th grade students of Taipei region, it could be found that the internal consistency coefficient of GPST was 0.90. The content validity, consistency validity, and construct validity of GPST were satisfactory. By way of principal component analysis, there was one factor— “the ability of genetics problem solving” could be extracted. To sum up all the above results indicated that the GPST is a suitable instrument for the assessment of genetics problem solving processes.