- 期刊
Synonymous Codon Usage in Marine Pico-Cyanobacterium Synechococcus sp. WH8102
海洋超微藻Synechococcus sp. WH8102基因體同義密碼子的使用
摘要
單細胞藍綠藻Synechococcus爲海洋生態系裡無所不在、數量多與高競爭性的植物性浮游生物，吾人可預期天擇的塵力對這類藍綠菌的演化相當重要。在本研究中，吾人研究海洋超微藻Synechococcus sp.WH8102基因體同義密碼子的使用。由於這藻種的核酸有較高GC組成(59.41%)，因此吾人預期這藻種之整體同義密碼子使用較多的以G或C結尾之密碼子。以多變數分析件（相關分析）Synechococcus sp. WH8102基因之相對同義密碼子(RSCU)，發現高表現與低表現基因分別分群在第一主軸的相反兩邊，且由高表現基因所找到此微生物的21個最佳密碼子皆爲以G或C結尾。整體而言，對於Synechococcus sp. WH8102同義密碼子使用來說，轉譯天擇與差異突變兩者皆是重要的影響因子，而疏水性則爲次要的影響因子。
並列摘要
Unicellular cyanobacteria Synechococcus are ubiquitous, abundant, and extremely competitive phytoplankton in the marine ecosystem. It may be expected that selection pressure would be important during the evolution of these cyanobacteria. In this study, synonymous codon usage in the genome of marine picoplankton Synechococcus sp. WH8102 was analyzed. The overall codon usage pattern indicates that G-or C-ending codons are predominant in Synechococcus sp. WH8102 because this organism is a GC rich organism (59.41%). However, highly-and lowly-expressed genes were found to cluster at opposite ends of the first major axis using correspondence analysis of WH8 102 genes based on relative synonymous codon usage (RSCU). Moreover, all of the 21 optimal codons found in highly -expressed genes ended with G or C. The results of this study indicate that both translational selection and mutational bias are crucial factors and hydrophobicity is a minor factor in shaping the codon usage pattern of Synechococcus sp. WH8102.