熱緊迫蛋白質70基因鼠在運動衰竭後其間質膠原酵素(MMP)活性的表現

Activity of Matrix Metalloproteinases (MMPs) in HSP70 Transgenic Mice after Exhaustive Exercise

秦作威(Trow-Way Chin) ； 林雋毅(Chuan-Yi Lin) ； 邱婉惠(Wan-Huei Chiou) ； 廖美華(Mei-Haw Liao) ； 卓奕宇(Yi-Yu Cho) ； 高婷玉(Ting-Yu Kao) ； 劉介仲(Chiuh-Chung Liu) ； 溫小娟(Hsiao-Chuan Wen)

《加馬》 41期 (2010/06) Pp. 15-20

https://doi.org/10.30173/JG.201006.0003

摘要

熱緊迫蛋白質(heat shock proteins; HSPs)爲細胞或生物體遭受外和環境如高溫、化學藥物等刺激時，在體內產生用以防衛之一系列蛋白質。HSPs的功能是幫助細胞的修復。在最近研究報告中指出過表現HSP 70的基因轉殖小鼠在熱中風生成過程中是可抗高溫、循環性休克及大腦休克。故以直接的基因轉殖方式產出的HSP70的確是有保護的作用。基質金屬蛋白酶(Matrix Metalloproteinases; MMP)其主要功能是分解細胞間質(matrix)，它主要的功能是在胚胎發育、形態生成…等過程中扮演便組織結構適時地重組成符合生物體的需求。MMP-2具有修復的功能。MMP-9對於細胞外基質中所有的成分也都具有分解的能力、通常在發炎狀態時由巨噬細胞、嗜伊紅血球、淋巴細胞等所表現，因此MMP-9的表現是代表組織處在發炎狀態。本篇報告之目標主要在探討：基因轉殖小鼠在誘導運動衰竭後其各器官（如腦、心臟、肺、腎、肝、肌肉、脾）的MMP-2及MMP-9活性變化。由此結果得知利用小鼠運動衰竭的模式中，得知全身性過表現HSP70的基因轉殖鼠其MMP-2活性的上昇升及MMP-9活性的下降是有助於了解HSP70基因轉殖鼠抗運動衰竭的。

關鍵字

熱緊迫蛋白質70 ； 基因轉殖小鼠 ； 基質金屬蛋白酶 ； 運動衰竭

並列摘要

Animals exposed to heat or other environmental insults express a set of highly conserved proteins known as heat shock proteins (HSPs) in many tissues. The HSPs are thought to be involved in cellular repairs, resulting in protection of the circulatory shock and other tissues from subsequent lethal damage. In present reports, transgenic mice overexpressing HSP7O in multiple organs improves survival during heatstroke by reducing hyperthermia, circulatory shock, and cerebral ischemia and damage in mice. In this study, we use the transgenic mice overexpressing HSP70 to explore the protective relationship between HSP70 and Matrix Metalloproteinases (MMPs) in exhaustive exercise. Exercise is induced by treadmill training. The MMPs play an important role in tissue remodeling associated with various physiological and pathological processes such as morphogenesis, angiogenesis, tissue repair, cirrhosis, arthritis and metastasis. MMP-2 and MMP-9 are thought to be important in metastasis. We study the MMP-2 and MMP-9 activity in various organs (including brain, heart, lung, kidney, liver muscle) in [+]HSP70(transgene-positive) and [-]HSP70(transgene-nagative) with or without exercise adaptation. These results demonstrate that the mouse exhaustion model fulfilles overexpression of HSP 72 in multiple organs improves MMP activity after acute exhaustive exercise.

並列關鍵字

Heat Shock Protein70 HSP 70 ； Matrix Metalloproteinases MMPs ； transgenic mice ； exhaustive exercise