With improvements in computer technology, noninvasive virtual endoscopy provides 3-dimensional (3-D) anatomical information by reconstructing 2-D imaging data from computed tomography or magnetic resonance imaging. Virtual endoscopy is particularly helpful for the assessment of the airway. We present a 17-year-old man who had multiple airway stenoses due to mucopolysaccharide deposition supraglottically and on the tracheal wall. Due to the potential risks of rigid endoscpy and general anesthesia, virtual endoscopy was arranged for the patient. Virtual endoscpy allowed not only for accurate presurgical evaluation of the airway anatomy, but also provided information for future bronchoscopic procedures such as placement of tracheobronchial stents. Virtual endoscopy identified a supraglottic mass caused by mucopolysaccharide depositioin, a circumferential stenosis at the glottic level due to previous CO2 laser vaporization, and tracheal wall stenosis. Virtual endoscopy provided a good simulation of endoscopy. The advantages, disadvantages, potential indications, and future roles of virtual endoscopy are discussed.