The main clinical feature of myasthe-nia gravis is that the fatigue consequent on muscular effort can be eliminated by rest or injection of cholinesterase inhibitors. We employed impedance audiometery to determine the manifestation of myasthenia gravis as well as its impact on the middle ear muscle in three patients. Prior to the injection of neostigmine, all three patients displayed C type tympanogram and no clearly observable stapedius reflex. The tympanogram turned to A type and the stapedius reflex resumed normal amplitude either 15 minutes after injection of neostigmine or 60 minutes after oral intake of Mestinon. A decline I nstapedius reflex was also noticed. Therefore, impedance audiometery is a trustworthy method for the diagnosis of myasthenia gravis because of the objective result and its easy application.