The shock absorber length corresponding to the most comfortable ride in bicycles is sought, with high school students being the target group. Based on existing travel comfort indices in railway travel, transmissibility, and natural frequency analysis in two degree-of-freedom systems, we carried out experiments on a scaled model of a bicycle frame. The frame model is excited using sine and pulse waves to quantify the effect of shock absorber length on level of vibration. At last, data acquisition and questionnaire study were carried out with an actual bicycle. It is found that the damping coefficient increases with a longer shock absorber, but the questionnaire results indicate that the respondents favour the bicycle with a shorter absorber. Besides sampling error, it is believed that the shock absorber length changes the spectrum of vibration at the seat. We believe that the Sperling's Ride Index used in the rail industry can describe cycling comfort qualitatively.