An Effective Production Method of Glucoamylase in a Novel Solid-State Culture System

新的固態醱酵系統提高葡糖糖化酶的生產效率

Tetsuo Kobayashi ； Teruyuki Nagamune ； Isao Endo ； Shinko Pantec

《Transactions of the Mycological Society of Republic of China》 6卷3&4期 (1991/12) Pp. 42-44

https://doi.org/10.7099/TMSRC.199112.0042

摘要

將明膠做成多孔之urethane型式並切成5mm邊長之立方塊(UFC)作為液態培養時Aspergillus oryzae B-1之生長載體以生產葡糖糖化酶。每克UFC可吸收19ml的培養基；將8g UFC投於850ml具有棒狀槳攪拌及壓榨板之反應槽(Bulk volume: 480ml)中，在醱酵後以擠壓方式將酵素液擠出，再投以新鮮培養基作為可重覆之批式醱酵系統。在糖濃度35g/l及濕度保持在80%，可獲得1189400u/l(unit: 10mg glucose production/30min at 40℃)之葡糖糖化酶，對糖收率為26u/mg, 分別為傳統固態醱酵之5及2.5倍。

並列摘要

Soft and porous material of urethane foams as carriers (UFC), which were impregnated with a liquid medium, were studied an effective cultivation system of Aspergillus oryzae B-3. Gelatin modified urethane foam was used by cutting into 5mm in side length cubes. We have impregnated 1g of the UFC with 19ml of this liquid medium. The total volume of this reactor was 850ml. Eight g of the UFC, whose bulk volume was about 480 ml, was taken into the new bioreactor. After appropriate time of initial batch cultivation, the liquid containing enzyme was squeezed from the UFC by pressing the squeezing plate. Then, the fresh medium was introduced into the reactor. The maximum production activity of glucoamylase was attainable under the condition where the initial concentration of sugar was around 35,000mg/l and the moisture content was kept about 80%. We have succeeded to produce glucoamylase about 5 times higher in the total activity and 2.5 times higher in the yield for sugar than they are producible by the conventional solid-state fermentation method.