Experimental Pancreatic Carcinogenesis in Syrian Golden Hamsters-A Valuable Animal Model for Studying Human Pancreatic Carcinogenesis

人類的胰臟癌一直難以早期診斷，為了瞭解胰臟癌自萌芽到增長的過程，吾人需藉助於動物實驗之模式。N-nitrosobis-2-oxopropylamine (B. O. P.)是目前被認為最具胰臟親和力的致癌原，因此，吾人採用B. O. P. 以誘潑倉鼠之胰臟癌，藉此觀察其癌之生成過程、病理變化。吾人以40隻雌性敘利亞倉鼠接受致癌原B. O. P.之皮下注射，每過一次，每次每公斤體重注射10毫克，速續注射6週。自注射後每隔2周犧牲數隻倉鼠以觀察致癌過程之系列性變化。33隻倉鼠為對照組，以生理食鹽水進行相同方式注射，並在相同時間內檢視倉鼠之病理變化。40隻注射B. O. P.之倉鼠共有17隻(42.5%)發生胰臟腺瘤，18隻(45%)發生胰臟腺痛，23隻(57.5%)發生胰臟腫瘤（包括腺瘤及腺癌）。33隻對照組則無一隻發生胰臟腫瘤。不論腺瘤或腺癌，其發生頻率均隨注射之時間而增加，但腫瘤之大小與注射時間長短未呈平行關係。胰臟腺瘤共44個，每個平均面積為0.61mm^2，胰臟腺癌共32個，每個平均面積為4.7mm^2。良性腺瘤以管狀腺瘤最多（40個），其次為乳頭囊狀腺瘤（2個）。惡性腺癌以管狀腺癌最多（22個），其次為乳頭管狀腺癌（5個）及粘液性腺癌（3個）。注射B. O. P.後第10週左右，胰臟之細胰管開始增生，此等變化主要發生於胰島周圍及胰島內部；繼而大量增生相互融合，形成微囊狀腺瘤(microcystic adenoma)，接着有些微囊狀腺瘤之上皮出現異常分裂或轉形為腺癌，有些則形成腫瘤。以B. O. P.注射倉鼠產生胰臟腫瘤之模式與人類胰臟癌有許多相似之處，吾人利用此模式觀察胰臟癌之演變過程，有助於人類胰臟癌之診斷。

關鍵字

胰臟癌；倉鼠；致癌原

並列摘要

N-nitrosobis-2-oxopropylamine (B. O. P.) was injected subcutaneously to 40 female Syrian golden hamsters at a dose of 10 mg/kg/week for 6 weeks. Thirty-three female hamsters injected with physiologic saline in the same dose-time schedule served as controls. Two to four animals of each group were sacrificed at biweekly intervals for 22 weeks. Both B. O. P.-treated group and control group steadily gained their body weight until the 16th week when a difference of body weight became statistically significant (P＜0.05), and it became even greater (P＜0.01) at the 22nd week. Seventeen (42.5%) out of the 40 hamsters developed pancreatic adenomas, 18 (45%) developed Pancreatic adenocarcinomas, and 23 (57.5%) developed adenoma and/or adenocarcinoma. None of 33 control hamsters had any pancreatic tumor during this period. The incidence of tumor increased progressively after injection of B. O. P. but no correlation existed between the tumor size and the length of time after B. O. P. treatment. The average area of the 44 pancreatic adenomas was 0.61 mm^2, and that of the 32 adenocarcinomas was 4.7 mm^2 on micrometric counting. Most of the benign tumors were tubular adenoma, and the malignant tumors appeared as tubular adenocarcinoma. Microscopically, carcinogenic effect became detectable at 10 weeks after B. O. P. injection. Peninsular hyperplasia of pancreatic ductules appeared first, followed by cystic dilatation of pancreatic islets, microcystic adenoma, metaplasia of ductular cells into columnar epithelia, focal dysplasia, true neoplasia and subsequently frank adenocarcinoma with invasion to the surrounding tissues. This animal model simulated the human pancreatic cancer in many aspects, including the histologic types, biologic behaviors, multiplicity of cancers, and coexistence of preneoplastic and neoplastic lesions in the same pancreas. It is concluded that hamsters treated with B. O. P. provides a valuable model for the study of Pancreatic carcinogenesis in humans.

並列關鍵字

pancreatic carcinoma ； hamsters ； carcinogenesis

國際替代計量

Experimental Pancreatic Carcinogenesis in Syrian Golden Hamsters-A Valuable Animal Model for Studying Human Pancreatic Carcinogenesis

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