- 期刊
Biochemical Characteristics of Alpha Fetoprotein from Ascitic Fluid of Hepatoma
肝癌病患腹水中甲胎蛋白之生化特性研究
摘要
甲胎蛋白是某些內科領域腫瘤診斷上之重要指標，在一般成人正常值約為20ng/ml以下，而在肝細胞癌症中它的含量會明顯增加，本實驗利用肝癌病患腹水中提取並精製甲胎蛋白，精製的方法包含利用免疫層析精製甲胎蛋白，再以氣相層析分析儀作甲胎蛋白碳水化合物的側鏈成份分析，並以氨基酸分析儀決定甲胎蛋白的氨基酸含量，與以前發表過的文獻數據作了比較，本實驗的結果顯示Glutamic acid的含量最高，糖鏈上含有五種碳水化合物，但是沒有發現N-Acetylneuraminic acid的存在，另外以SDS方法測得分子量約在67,0000。 同時我們將精製後的甲胎蛋白送請美國德州大學達拉斯醫學中心作了氨基酸的排序，得到的結果為RTLHRNEYGIASILDSYQCTA，前面10個氣塞酸序列與1996年Y Koyama等人的結果一致，將有助於我們對甲胎蛋白的生化特性之瞭解，並作為今後肝癌患者流行病學研究的重要參攷。
並列摘要
In this study, we successfully purified the carbohydrate moiety of the alpha fetoprotein from ascites fluid of hepatoma patients by immunoaffinity chromatography and gel filtration chromatography. The results of the amino acid composition analysis showed that Glu had the highest proportion. Mannose, Galactose, Glucose, N-Acetylglucosamine and Sialic acid. were found in the alpha fetoprotein from hepatoma patients. No N-Acetylneuraminic acid was found in this study, even though it is an amino sugar content presenting in bovine alpha fetoprotein. The absence of N-Acetylneuraminic acid of alpha fetoprotein from hepatoma patients is probably due to the fact that the quantity was too low to detect. A molecular weight of 67,000 was found by SDS polyacrylamide. The amino acid sequence of this purified alpha fetoprotein, analyzed by the Department of Microbiology, School of Allied Health Science, the University of Texas Health Science Center at Dallas is RTLHRNEYGIASILDSYQCTA; the results of the first 10 amino acid sequence are in good agreement with the results made by Y. Koyama, K. Taketa, M. Azuma et al. in 1996. Eventually we will provide the alpha fetoprotein's data as a standard reference for clinical laboratories and research laboratories for other studies such as epidemiological studies of hepatocellular carcinoma in Taiwan.
延伸閱讀
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