Splenic abscess is an uncommon clinical entity. In untreated case the mortality rate is very high which had been attributed to delayed clinical diagnosis. Multiple splenic abscesses have higher mortality than that of solitary abscess and are usually diagnosed after autopsy. Tuberculosis is a rare causative organism of splenic abscess as compared with other pyogenic organism. Here we report a case of multiple tuberculous splenic abscesses diagnosed by real-time ultrasonography and fine-needle aspiration and confirmed by culture and pathology after splenectomy. The patient was a 13 years old boy who suffered from acute myelogenous leukemia and had been treated with chemotherapy. Severe leukopenia and persistent high fever lasting for more than one month were noticed after chemotherapy. Real-time abdominal sonography showed hepatosplenomegaly and a small hypoechojc lesion in the spleen which was misdiagnosed. One week later the patient had sudden onset of abdominal pain and presence of ascites. Follow-up abdominal sonography showed multiple hypoechoic lesions associated with posterior acoustic enhancement in the enlarged spleen. Fine-needle aspiration of the splenic lesion under echo-guidance was performed and some yellowish caseous material was aspirated out which showed positive acid-fast stain. After splenectomy, Mycobacterium tuberculosis was isolated from the spleen by culture. The patient recovered gradually after anti-tuberculous therapy.