We retrospectively examined the prognostic importance of tumor cell DNA content (ploidy) to the survival of patients with primary colorectal carcinoma. The DNA content of each tumor was determined by flow cytometry on nuclei isolated from paraffin-embedded tissues. In 37 of the 171 tumors investigated, the histogram was not analyzable. Of the remaining 134 cases, 94 (70%) displayed near-diploid and 40 (30%) were aneuploid tumors. No association was found between the tumor DNA ploidy status and sex, age, pathological stage, histological grade, or pre-operative CEA levels. Tumors located at the cecum and ascending colon were more frequently diploid than those at other sites (p<0.05). The median survival time of the patients with aneuploid tumor was 36months, and was not reached for those with near-diploid tumor (p<0.002). The subgroups of patients in which tumor DNA ploidy exerted an influence on survival were: patients with stage. B tumors (p<0.02), those with moderately differentiated tumors (p<0.02), those with transverse colon tumors (p<0.05), and patients >40 years-old (p=0.01). Muirivariant analysis showed that pathological stage, ploidy, and age of patient (≤40 v.s>40 years-old) were all independent prognostic factors, whereas histological grade and pre-operative CEA level were not. Our results indicate that the measurement of rumor DNA ploidy is a useful adjunct in assessment of prognosis in patients with primary colorectal cancer especially for those classified as ”intermediate risk” by conventional clinicopathological parameters.
We retrospectively examined the prognostic importance of tumor cell DNA content (ploidy) to the survival of patients with primary colorectal carcinoma. The DNA content of each tumor was determined by flow cytometry on nuclei isolated from paraffin-embedded tissues. In 37 of the 171 tumors investigated, the histogram was not analyzable. Of the remaining 134 cases, 94 (70%) displayed near-diploid and 40 (30%) were aneuploid tumors. No association was found between the tumor DNA ploidy status and sex, age, pathological stage, histological grade, or pre-operative CEA levels. Tumors located at the cecum and ascending colon were more frequently diploid than those at other sites (p<0.05). The median survival time of the patients with aneuploid tumor was 36months, and was not reached for those with near-diploid tumor (p<0.002). The subgroups of patients in which tumor DNA ploidy exerted an influence on survival were: patients with stage. B tumors (p<0.02), those with moderately differentiated tumors (p<0.02), those with transverse colon tumors (p<0.05), and patients >40 years-old (p=0.01). Muirivariant analysis showed that pathological stage, ploidy, and age of patient (≤40 v.s>40 years-old) were all independent prognostic factors, whereas histological grade and pre-operative CEA level were not. Our results indicate that the measurement of rumor DNA ploidy is a useful adjunct in assessment of prognosis in patients with primary colorectal cancer especially for those classified as ”intermediate risk” by conventional clinicopathological parameters.