Expression of Aspartic Proteinases in Gastric Cancer: An Immunohistochemical Study

以免疫組織化學染色法測定胃癌天門冬氨酸蛋白酶分佈情形

黎國洪(Kwok-Hung Lai) ； 王覲瑜(Jiin-Yu Wang) ； 陳銘山(Ming-San Chen) ； 莫景棠(King-Tong Mok) ； 曾暉華(Huey-Hwa Tseng)

《中華民國消化系醫學會雜誌》 11卷3期 (1994/09) Pp. 194-200

https://doi.org/10.6557/GJTa.199409_11(3).0003

摘要

本研究以免疫組織化學染色法來測定45個胃癌手術標本中第一型胃蛋白酶原(PGⅠ), 第二型胃蛋白酶原(PG Ⅱ)及組織蛋白酶E(Cathepsin E)分佈狀況。在正常的胃上皮或腺體中，測PGⅠ、PGⅡ及Cathepsin E之比率分別為46.7%、88.9%及97.5%。在兩個胃癌標本中，只有幾個癌細胞可測出PGⅠ(4.4%)，而PGⅡ及Cathepsin E則散在性或瀰漫性分佈在癌細胞中，其陽性比率分別為PGⅡ 26.7%及Cathepsin E 66.7%。各蛋白酶之分佈和癌細胞原發位置及分化無關。在陽型上皮胃癌(Gastric cancer of intestinal type)併發轉移及無腸化生之標本，PGⅡ陽性率有較高傾向，但衹有無腸化生標本之PGⅡ較高陽性率具統計學意義。在45個標本中單獨Cathepsin E陽性19個，Cathepsin E及PGⅡ陽性9個，三種蛋白酶均陽性2個，單獨PGⅡ陽性2個，蛋白酶陰性者14個。從結果顯示，國內的胃癌細胞多源自表皮及小凹細胞，甚少從PGⅠ分泌細胞發生。同時，PGⅡ在有轉移的癌症，腸型腺癌(Intestinal type adenocarcinoma)及不具腸化生之標本出現率較高，其是否和癌症之散佈有關，有待進一步探討。

關鍵字

天門冬氨酸蛋白酶 ； 胃癌 ； 第一型蛋白酶原 ； 第二型蛋白酶原組織蛋白酶E

並列摘要

We used the immunohistochemical method to localize the aspartic proteinases, including pepsinogen Ⅰ (PG Ⅰ), pepsinogen Ⅱ (PG Ⅱ), and cathepsin E in 45 gastrectomy specimens from patients with gastric carcinoma. Positive staining for PG Ⅰ, PG Ⅱ and cathepsin E were 46.7%, 88.9% and 97.8% in normal glands or epithelial cells, and 4.4%, 26.7%, and 66.7% in cancer cells respectively. The distribution of PG Ⅱ and cathepsin E varied from diffuse to sporadic, but only a few cancer cells were positive for PG Ⅰ in 2 specimens. There was no relationship between the locations and differentiation of cancer and the positive staining of aspartic proteinases in cancer cells. Cancer without concomitant intestinal metaplasia had a higher incidence of PG Ⅱ staining than cancer with intestinal metaplasia (36.7% vs 6.6%, P=0.031). In addition, higher incidences of PG Ⅱ positive cancer cells were found in the intestinal type than in diffuse type carcinoma, with greater presence of metastasis than absence of metastasis, but their differences did not reach statistical significance. Among the 45 specimens, cancer cells were positive for the following: Cathepsin E only: 19 specimens; cathepsin E and PG Ⅱ: 9; both three aspartic proteinases: 2; PG Ⅱ only: 2. Cancer cells were negative for all three proteinases in 14 specimens. The distribution of aspartic proteinases in our gastric cancer indicated that most of those cancers arose from surface or foveolar epithelial cells, and rarely from PG Ⅰ producing cells. There is a tendency to have more PG Ⅱ staining in metastatic cancer, intestinal type adenocarcinoma and cancer not associated with intestinal metaplasia. Thus, the role of PG Ⅱ in carcinogenesis and cancer spreading needed further investigation.

並列關鍵字

aspartic proteinase ； gastric cancer ； pepsinogen Ⅰ ； pepsinogen Ⅱ ； cathepsin E