- 期刊
Determining the Genetic Signature of Oral Submucous Fibrosis by the Use of Laser Capture Microdissection and a cDNA Microarray
並列摘要
Oral submucous fibrosis (OSF) is a premalignant condition with a high potential to develop into oral cancer. Epidemiologic studies have suggested the habit of areca (Areca catechu) quid chewing to be a major etiologic factor of OSF. The most prevalent location for OSF is the buccal mucosa, rendering this location the most common oral cancer site in Asian populations. In this study, we used laser capture microdissection to obtain oral epithelium for cDNA microarray analysis, and identified gene candidates that are differentially regulated between normal and OSF oral epithelia. We found 109 genes that are upregulated in OSF epithelium, including cyclins, tumor and suppressor candidate 3 (TUSC3) and 4, and BPA1. Another 169 genes were downregulated in OSF epithelium, including retinoic acid induced 3, small GTPases, and apoptosis-related Harakiri. Among these, the expression of TUSC3 in OSF epithelium was validated by real-time reverse-transcriptase polymerase chain reaction. Further investigations are needed to discover the roles of these candidate genes in the malignant transformation of OSF epithelium into oral cancer.
延伸閱讀
- Muff, R., Kumar, R. M. R., Botter, S. M., Born, W., & Fuchs, B. (2012). Genes Regulated in Metastatic Osteosarcoma: Evaluation by Microarray Analysis in Four Human and Two Mouse Cell Line Systems. Sarcoma, 2012(), 653-665. https://doi.org/10.1155/2012/937506
- Lin, C. Y., Chen, H. A., Chen, C. H., Liao, H. T., Wang, H. P., & Chou, C. T. (2005). Application of cDNA Microarray to Measure the Effect of Tripterygium Wilfordii Hook F (TWH) on the Mononuclear Cytokine, Apoptotic, and other Inflammatory Gene Expressions in Patients with Ankylosing. Formosan Journal of Rheumatology, 19(3&4), 107-114. https://doi.org/10.6313/FJR.2005.19(3-4).05
- 林雪芳（2008）。Identification of insect cell lines and cell line cross-contamination (CLCC) by internal transcribed spacer region of ribosomal DNA〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2008.02114
- Peck, K. (2000). DNA Microarray with Colorimetric Detection for Gene Expression Analysis. Journal of Genetics and Molecular Biology, 11(3), 114-116. https://doi.org/10.30047/JGMB.200009.0005
- 王佾柔（2005）。Application of cDNA Microarray Technique for Gene Expression Analysis in Arecoline treated Human Oral Gingival Fibroblasts〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0011-2903200614044868