Traditional investigations using analytical approaches on the boundary conditions of vibrating plats by are very complicated. Meanwhile, the increasing number of boundary parameters with finer meshing by finite element modeling degrades the accuracy of identification results. This research work identifies the multiple boundary parameters of vibrating plates at a single frequency by using genetic algorithm method, combined with finite element modeling and frequency response functions of vibration experiments. Plates clamped by wedge-lock retainers are investigated, and the results show that the identification process proposed in this work is effective and could be applied in industrial cases.