A study of thermophoresis and radiation effects on heat and mass transfer from an unsteady stretching surface is reported. In this work, air flow was modeled as two dimensional, incompressible laminar flow. The similarity transformation and numerical technique are used to solve governing equations include mass, momentum, energy and concentration. The solutions are obtained by numerical method of Runge-Kutta integration with shooting scheme. The results include velocity, temperature and concentration distribution for different unsteadiness parameter A, radiation parameter R, and thermophoretic parameter τ. It showed that the velocity profiles decrease with increasing A values. The heat transfer rate increased with increasing A and R values. The effects of thermophoresis is particularly important for concentration blowing away from a hot surface at a given temperature gradient.