Synthesis of Amyloses of Controlled Molecular Weight Using Rabbit Phosphorylase A

運用酵素合成高得率高純度之直鏈澱粉

張慎餘(Shen-Yu Chang)

《華岡工程學報》 9期 (1995/07) Pp. 261-275

https://doi.org/10.29987/CCUHKJE.199507.0016

摘要

文獻報告中雖然已經有合成直鏈澱粉的成功經驗，但是其得率均在毫克的範圍，且其聚合度在幾百之間。本研究運用酵素開發出一新的合成步驟，將直鏈澱粉的得率提升至一克的範圍，同時將其聚合度提高到六千以上。另外，研究結果證明，合成好的直鏈澱粉不含有任何枝鏈澱粉，且其分子量分佈很窄。除此之外，本研究亦發現，針對己衰變且難溶的澱粉，可運用溶劑二甲基氧化硫先加以溶解，再進行分子衍生，以利高分子分子量的分析。

關鍵字

直鏈澱粉 ； 合成 ； 酵素 ； 高分子 ； 分子量

並列摘要

Although the literature has shown that linear amyloses can be synthesized using enzymic techniques, the yields are only in the milligram range and the DPs in the hundreds. Using commercially available reagents, this study has developed a means of synthesizing amyloses with DPs up to 6100 in quantities approaching one gram. The synthesized amyloses are free of α-1.6 branches and have comparatively narrow molecular weight distributions. Additionally, this study demonstrates that DMSO is applicable for derivatizing amyloses to their carbanilates before gel permeation chromatographic analysis. The procedure developed is useful for derivatizing retrograded amyloses having limited solubility in pyridine. Furthermore, when DMSO is used, low temperature (-15℃) must be maintained to prevent amyloses from depolymerization.