In this article, use alkaline pulp and cellulose powder to experiment. The first is adding the fiber hydrolase and samples into the Erlenmeyer-flask for saccharification process for seventy-two hours. In this process, should conduct brix-measurements every twenty-four hours and activation of the yeast after the saccharification process. Then add yeast for sugar into ethanol for the fermentation process for seventy-two hours. After seventy-two hours, put the samples into centrifuge. Take samples after centrifugation at the top of the clear liquid to test sugar content. First to ethanol were analyzed and to analyze out data as a Calibration standard. Use standards of samples to analysis and calibration to judge by the samples of the ethanol concentration.