奈米粒子強化之超高解析度表面電漿共振生物感測器

Nanoparticle-enhanced ultrahigh-resolution Surface Plasmon Resonance Biosensors

簡汎清(F. C. Chiena) ； 黃崑財(K.-T. Huang) ； 林俊佑(G. Y. Linb) ； 陳顯禎(S.-J. Chenc)

《光學工程》 84期 (2003/12) Pp. 1-8

https://doi.org/10.30011/OE.200312.0001

摘要

本文設計與製造一種嶄新含有金奈米粒子強化(Au nanoparticle-enhanced)之表面電漿共振(surface plasmon resonance, SPR)生物感測器(biosensor)，經實驗證實其有機會偵測至100fg/m平方公尺生物分子表面覆蓋度之超高解析度，將可簡化生物分子數量放大之程序(如polymerase chain reaction, PCR)。此生物感測器事先不須額外標記(label-free)，能直接分析微量生物分子間交互作用分析(biomolecular interaction analysis, BIA)，可應用至到新藥研發、基因定序、醫療診斷等領域上，預期將成為極重要的生物篩檢感測器。

關鍵字

奈米粒子強化 ； 表面電漿共振 ； 生物感測器 ； 生物分子間的交互作用分析

並列摘要

The present study develops a novel nanoparticle-enhanced ultrahigh-sensitivity Surface Plasmon Resonance (SPR) biosensor which offers a ten-fold improvement in sensitivity when compared to conventional SPR biosensors in the detection of the surface coverage of biomaterials. The proposed biosensor employs the Attenuated Total Reflection (ATR) method to excite the Surface Plasmon Wave (SPW) and relies upon the careful control of the size and volume fraction of the embedded Au nanoparticles to increase the sensitivity of the device without the requirement for extrinsic material labeling. The SPR biosensor design is based upon the Maxwell-Garnett model and the Fresnel equations, and the subsequent fabrication of the device is performed using a co-sputtering deposition system. The current results indicate that the developed nanocluster-enhanced ultrahigh-sensitivity SPR biosensor has the clear potential to analyze tiny biomolecular interactions directly without the need for additional biomolecular labeling. Consequently, this form of SPR biosensor is destined to become one of the most important sensing devices in the field of biomolecular diagnosis.

並列關鍵字

nanoparticle-enhanced ； surface plasmon resonance ； biosensor ； biomolecular interaction analysis