應用於多焦面光學螢光切片影像之光斑調變體積全像顯微鏡

結構式照明顯微鏡已被廣泛應用於重建寬視野的光學切片螢光影像，然而，過往的技術皆需要對不同深度做掃描來獲得多層的光學體積影像。在本研究中，我們發展一套新的技術，稱之為光斑體積全像顯微術。此技術結合了結構式光斑照明以及體積全像光柵，可用於截取並同時重建多層的光學切片影像，且無須對不同焦平面做掃描。我們建構此一系統，並對螢光小球樣本及小鼠小腸樣本做驗證，成功的擷取多焦面之光學螢光切片影像。

關鍵字

體積全像術；三維顯微鏡；光斑影像

並列摘要

Structured illumination microscopy has been widely used to reconstruct optically sectioned fluorescence images in wide-field fashion; however, it still requires axial scanning to obtain multiple depth information of a volumetric sample. In this paper, a new imaging scheme, called speckle-based volume holographic microscopy system, is presented. The proposed system incorporates volumetric speckle illumination and multiplexed volume holographic gratings to acquire multi-plane images with optical sectioning capability, without any axial scanning. We present the design, implementation, and experimental image data demonstrating the proposed system's ability to simultaneously obtain wide-field, optically sectioned, and multi-depth resolved images of fluorescently labeled microspheres and tissue structures.

並列關鍵字

Volume holography ； Three-dimensional microscopy ； Speckle imaging

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