黑殭菌低溫噴霧乾燥微膠囊化之研究

Study on Low-Temperature Spray Drying for the Microencapsulation of Metarhizium anisopliae

劉清標(Ching-Piao Liu)

《中華科技大學學報》 45期 (2010/10) Pp. 77-86

https://doi.org/10.7095/JCUST.201010.0077

摘要

本試驗方法採低溫噴霧乾燥方式，進行黑殭菌孢子之微膠囊化，目的使菌孢維持活性與延長保存期限。包覆材料利用HPMC、幾丁質、糊精、脫脂奶粉和PVPK90等不同材質，探討包覆效果。依不同入風口／出風口溫度，輸送系統組成與孢子濃度等條件來探討孢子之存活率。另外也分析樣品之特性如粒徑大小分佈，包覆率與形狀等。實驗利用10%脫脂奶粉、10%糊精與5% PVP k90包覆之比例，可得到最好之包覆效果，菌株孢子存活率可維持80%。實驗如單以12%糊精配方，整體產品回收率可提高到85%，平均顆粒大小分佈在2.5~10μm之間。經噴霧乾燥之粉末表面成平滑或皺摺狀，由結果得知黑殭菌孢子經低溫噴霧乾燥微膠囊包覆後，雖經半年後之4℃儲藏期間，活性仍可維持80%以上。

關鍵字

低溫噴霧乾燥 ； 黑殭菌 ； 微膠囊化 ； 孢子

並列摘要

Low-temperature spray drying was used to microencapsulate the conidia of Metarhizium anisopliae. In order to prolong the activity of the conidia and improve their shelf life, various polymers such as HPMC, chitosan, dextrin, skim milk and PVP K90 were examined as encapsulation matrices. Different inlet/outlet temperatures, compositions of the carrier systems, and conidia concentrations were examined with respect to spore viability. Moreover, the production characteristics, such as particle size distribution, entrapment efficiency, and shape, were investigated. The best encapsulated products were obtained using a matrix composed of 10% dextrin, 10% skimmed milk, and 5% PVP K90 as the coating material. The spore viability was 80%, whereas that with dextrin only as coating material the yield was 85±3, The microspheres obtained by spray drying were spherical with smooth or wrinkled surfaces, the mean particle size was in the range of 2.5~10μm. The conidia exhibited 80% spore viability, even after storage for 6 months at 4℃.

並列關鍵字

Low-temperature spray drying ； Metarhizium anisopliae ； Microencapsulation ； Conidia