蠶絲蛋白／尼龍6奈米纖維薄膜的製備與特性研究

本研究將蠶絲蛋白與尼龍6，運用靜電紡絲加工技術來製備奈米纖維薄膜，並探討蠶絲蛋白（10% w/v）與尼龍6（12% w/v）混摻溶液之不同比例濃度對成絲影響，以製作最佳化的蠶絲蛋白／尼龍6奈米纖維薄膜。並利用場放射型掃描式電子顯微鏡（SEM）、傅立葉轉換紅外線光譜分析儀（FTIR）及拉力試驗，分別探討產物之表面型態、化學結構、機械性質等相關性質。研究結果顯示，FTIR光譜顯示蠶絲蛋白經由丙酮處理後，其分子構象由無規捲曲轉變成β－摺板。蠶絲蛋白／尼龍6奈米纖維薄膜之平均纖維直徑與拉伸強力隨著尼龍6含量增加而增加。使用MTT分析法進行纖維母細胞L929在蠶絲蛋白／尼龍6奈米纖維薄膜上貼附及增生情況評估，實驗結果顯示無毒性及具有良好的生物相容性。由上述實驗結果顯示蠶絲蛋白／尼龍6奈米纖維薄膜在生醫材料之創傷敷料的領域上有極大的潛力。

關鍵字

靜電紡絲；蠶絲蛋白；尼龍6 ；創傷敷料

並列摘要

Silk fibroin (SF) and nylon 6 nanofibrous mats were fabricated via electrospinning process. Solutions of 10% w/v SF with 12% w/v nylon-6 were blended in different weight ratios and electrospun into nanofibers. The resulting mat was further rearranged from random coil to β-sheet structure of SF through acetone-treating. The morphology, structure and mechanical properties of the obtained electrospun fibrous mats were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and tensile testing, respectively. Infrared spectra showed that the β-sheet structure of the silk fibroin increased after acetone treatment. SEM images and tensile testing showed that the diameter and tensile strength of the SF/nylon 6 nanofibrous mats were mainly affected by the weight ratios of the blend solutions. The attachment and proliferation properties of L929 fibroblast cells on the SF/nylon 6 nanofibers were analyzed by the MTT assays. Cytotoxicity tests of the SF/nylon 6 nanofibers showed that the fibers were non-toxic to L929 fibroblast cells. Therefore these results proposed that the SF/nylon 6 nanofibers may be used for biomedical materials such as wound dressing.

並列關鍵字

electrospinning ； silk fibroin ； nylon 6 ； wound dressing

國際替代計量

蠶絲蛋白／尼龍6奈米纖維薄膜的製備與特性研究

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