幾丁聚醣／槲皮素混摻水膠之製備及應用

Preparation and Application of Chitosan/Quercetin Blend Hydrogels

饒文娟(Win-Chun Jao) ； 徐敏容(Min-Jong Hsu)

《中華科技大學學報》 64期 (2015/10) Pp. 135-143

摘要

槲皮素是一種黃酮類天然的抗氧化劑；具有多種生物活性，如抗發炎、抗病毒、抗癌和抑菌等。本研究將幾丁聚醣與醋酸纖維素混摻製備成水膠，並在水膠內包覆不同比例槲皮素。幾丁聚醣／醋酸纖維素水膠在不同pH值的緩衝液測試其膨潤性質，實驗結果顯示幾丁聚醣／醋酸纖維素水膠隨著pH值增加其膨潤度減少。幾丁聚醣／醋酸纖維－槲皮素水膠其抗氧化化活性以DPPH自由基清除率、亞鐵離子螯合能力與總酚含量等分析方法探討。最後以L929小鼠纖維母細胞觀察其生物毒性，其結果顯示混摻水膠未具有細胞毒性。綜合以上結果，幾丁聚醣／醋酸纖維－槲皮素水膠做為傷口敷料，極具有發展潛力。

關鍵字

幾丁聚醣 ； 醋酸纖維素 ； 槲皮素 ； 膨潤行為 ； 抗氧化活性

並列摘要

Quercetin, a flavonoid type natural antioxidant, exhibits various biological effects, such as anti-inflammatory, anticancer, antiviral and antibacterial activities. In this study, chitosan (CS)/cellulose acetate (CS) blended hydrogels with various content of quercetin were prepared. The swelling behaviour of the chitosan/cellulose acetate (CS/CA) hydrogels was examined in buffered solutions at various pHs. The equilibrium swelling ratios of the CS/CA hydrogels decreased with increasing pH. The antioxidant activities of quercetin-loaded CS/CA hydrogels were evluated by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ferrous iron chelating and total phenolic content assays. In addition, the quercetin-loaded CS/CA hydrogels were proven non-cytotoxic to L929 mouse fibroblasts. These characteristics indicated that the quercetin-loaded CS/CA hydrogel could be a promising canditdate for wound dressing.

並列關鍵字

chitosan ； cellulose acetate ； quercetin ； swelling behaviour ； antioxidant activity