含薑黃素之蠶絲蛋白奈米纖維膜其生物相容性評估

薑黃素其藥理活性眾所皆知，包括傷口癒合、抗氧化、抗發炎、抗菌及抗腫瘤等特性。本研究將蠶絲蛋白添加薑黃素利用靜電紡絲方式來製備奈米纖維膜，此奈米纖維膜經由丙酮改質後可提升蠶絲蛋白其β-sheet 結晶態比例。由掃瞄式電子顯微鏡及雷射掃描式共軛焦顯微鏡觀察此蠶絲蛋白及蠶絲蛋白/薑黃素奈米纖維膜表面型態及螢光性質，其奈米纖維膜的纖維形貌均勻，纖維平均直徑約在490 到520 nm 之間。蠶絲蛋白/薑黃素奈米纖維膜之抗氧化活性以DPPH 自由基清除能力分析其值為87.8 %。並探討此奈米纖維薄膜在37 ◦C 下醋酸緩衝液/甲醇之薑黃素釋放行為，其釋放動力學符合Fickian diffusion 擴散機制。體外間接MTT 分析方法顯示絲蛋白/薑黃素奈米纖維膜對L929小鼠纖維母細胞具有良好的細胞相容性，在模擬傷口癒合試驗證明薑黃素之釋放能有效促進細胞遷移。實驗結果顯示此蠶絲蛋白/薑黃素奈米纖維膜在創傷敷料的領域上有極大的潛力。

關鍵字

靜電紡絲；蠶絲蛋白；薑黃素；細胞相容性；傷口癒合試驗

並列摘要

Silk fibroin (SF) nanofibrous mats containing curcumin, which is well-known for its pharmacological activities including wound healing, anti-microbial, antioxidant, anti-inflammatory and antitumor properties, were fabricated via electrospinning process and treated with acetone in order to induce the β‐sheet structure. The influence of curcumin on the morphology and fluorescent properties of nanofibrous matrices was investigated using scanning electron microscopy (SEM) and laser scanning confocal microscopy (LCSM). The morphology of the SF and SF/curcumin nanofiber mats were smooth, with the average diameter of the nanofibers ranging from 490 to 520 nm. The antioxidant activity of SF/curcumin nanofiber mats was determined through the free-radical scavenging ability of DPPH, with 87.8 %. The curcumin release behavior from the SF/curcumin nanofibrous mats was investigated in acetate buffer solution that contained methanol at 37 ◦C and the release kinetics of curcumin followed the Fickian diffusion mechanism. In vitro indirect MTT assay also showed that the SF/curcumin nanofiber mats have good cytocompatibility to L929 mouse fibroblasts. As shown by in vitro scratch assay, curcumin enhanced the migration of L929 fibroblasts. The results indicated that the SF/curcumin nanofibrous mats could be considered as candidates for wound dressings.

並列關鍵字

electrospinning ； silk fibroin ； curcumin ； cytocompatibility ； scratch assay

國際替代計量

含薑黃素之蠶絲蛋白奈米纖維膜其生物相容性評估

未授權

主題瀏覽