Abstract Recently, disease outbreaks had caused mass mortalities of laboratoryreared larvae of the ficus transparent wing moth, Perina nuda (Fabricius). Moribund larvae exhibited a syndrome similar to silkworms infected with infectious flacherie virus. Two viruses, P. nuda nucleopolyhedrovirus (PenuNPV) and a new picorna-like virus (named PnPV), have been isolated from infected larvae with flacherie disease. Negatively stained preparations showed that the virus exhibits icosahedral symmetry and measures 30 nm in diameter with no envelope and no distinct surface structures. The hemolymph of moribund larvae with flacherie disease is either with or without free polyhedra caused by the infection of PenuNPV, The oral-infection experiments showed that pathogenesis of the picorna-like virus is not obvious, but it may cause a synergistic effect in mortality and decrease the yield of polyhedra in infected P. nuda larvae with PenuNPV.
摘要 實驗室飼育之榕樹透翅毒蛾 (Perina nuda) 幼蟲發生大量死亡，瀕死幼蟲呈現類似家蠶軟化症 (flacherie) 之病徵。從呈軟症之榕樹透翅毒蛾幼蟲可以分離到兩種病毒，一種是榕樹透翅毒蛾核多角體病毒(Perina nuda nucleopolyhedrovirus, Penu-NPV)，另一種是小病毒。在負染法處理之下，此小病毒呈現正二十面體的對稱，直徑約為30nm，不具有被膜 (envelope) 和明顯的表面構造，屬小RNA病毒群種類，命名為PnPV。混合感染PenuNPV 之軟化症幼蟲，其血淋巴含有或不含有游離的多角體。經口感染實驗證實小病毒之病原性並不強，但可能對PenuNPV殺蟲而言有協力作用，而對利用幼蟲量產多角體上則有負面的影響。
| Key words: Perina nuda, flacherie, PenuNPV, insect, picorna-like virus. | | 關鍵詞: 榕樹透翅毒蛾、軟化症、核多角體病毒、昆蟲、小RNA病毒 |
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