Abstract The 16S ribosomal DNA (rDNA) of the secondary endosymbiont and Wolbachia-specific gene, wsp (cell surface protein of Wolbachia), were amplified and sequenced. Based on the occurrence of 16S rDNA and wsp by PCR, more than 55% and 38% of examined B. tabaci populations (n = 202) harbored secondary endosymbiont and Wolbachia, respectively. Molecular phylogenetic analysis of 16S rDNA sequences showed that the secondary endosymbiont was located in the γ-3 clade of a subdivision of the Proteobacteria. Wsp gene sequences revealed that the Wolbachia found in B. tabaci populations of Taiwan belong to group B and subgroup Con/Rug. The results also showed that the carrying/infection rate of secondary endosymbiont/Wolbachia might be related to the biotype of B. tabaci.
摘要 本研究對於臺灣地區所採集之202筆煙草粉蝨族群，利用PCR反應對於煙草粉蝨二級內共生菌之16S rDNA 及 Wolbachia 之表面蛋白基因 (Wolbachia surface protein, wsp) 的增幅，以進行內共生菌的偵測；結果顯示，臺灣地區55.4％的煙草粉蝨族群受二級內共生菌的感染，38.1％的族群受 Wolbachia 的感染，再經由類緣關係分析，感染臺灣地區煙草粉蝨之二級內共生菌皆為 Proteobacteria γ-3 亞綱的種類，而 Wolbachia 則屬於B群的 Con/Rug 亞群的種類。由各生物小種感染之比例顯示，此二類內共生菌之分布與生物小種有較密切的相關，然而影響其分布的因去仍需後續的研究以進一步釐清。
| Key words: Aleyrodidae, Bemisia tabaci, secondary endosymbionts, biotype, Taiwan | | 關鍵詞: 粉蝨科、煙草粉蝨、二級內共生菌、生物小種、臺灣 |
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