Abstract The whitefly, Bemisia tabaci, is an important agricultural pest and is globally distributed. Although the B. tabaci species complex includes many biotypes, they are morphologically indistinguishable. Therefore molecular markers must be used to identify them. In this study, B. tabaci specimens were collected from Taiwan and adjacent islands. Mitochondrial 16S rDNA sequences were used to reconstruct phylogenetic trees in order to distinguish the biotypes. According to the collection records, B. tabaci is widely distributed in Taiwan, Penghu, Green Island, Orchid Island, Matsu, and Kinmen. Phylogenetic analyses revealed that 3 biotypes exist in Taiwan: the B, Nauru, and An biotypes. We hope this study will contribute to the knowledge of damage by and virus transmission of B. tabaci in the future.
摘要 煙草粉蝨 (Bemisia tabaci) 為泛世界分佈的農業害蟲，包括許多生物小種 (biotype) 而構成一種群 (species complex)，但生物小種間不易利用形態特徵來區別，因此近年來利用分子標示 (molecular markers) 來進行鑑定。本研究全面採集臺灣地區之煙草粉蝨，並應用粒線體 16S rDNA 片段序列來進行系統發生之分析，根據系統發生樹結果來鑑定生物小種。結果顯示煙草粉蝨廣泛分佈於臺灣本島、澎湖、金門、馬祖、蘭嶼及綠島。此外，應用粒線體 16S rDNA 系統發生樹可明確區別各生物小種，並鑑別出臺灣地區有 3 生物小種，分別為 B、Nauru 和 An 生物小種。本研究明確鑑別臺灣地區煙草粉蝨之生物小種，期望有助於煙草粉蝨相關為害與傳播病毒之研究。
| Key words: Aleyrodidae, Bemisia tabaci, biotype, molecular marker, Taiwan | | 關鍵詞: 粉蝨科、煙草粉蝨、生物小種、分子標示、臺灣 |
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