隨著微生物對抗生素藥物的抗藥性發展， 抗菌性胜肽的開發及研究，是一項重要而嚴肅的課題。從許多生物天生的防禦機制中發現的抗菌胜肽對細菌與黴菌的抑制作用，提供了一個有效而新穎的方式。然而抗菌胜肽處於不同酸鹼度及不同鹽度的環境對微生物的抗菌效果，會影響抗菌性胜肽的殺菌效果,限制臨床使用的可行性。本研究是針對序列為Ac-KWRRWVRWI-NH2富含色氨酸 (Trp-rich) 的胜肽Pac-525，D-Nal-Pac-525及另一富含組氨酸的 胜肽 P113 (AKRHHGYKRKFH - NH2 ),對格蘭氏陽性(Gram positive)，格蘭氏陰性(Gram negative)細菌及真菌(fungus)的抗菌效用進行研究。本研究使用核磁共振儀來測定P-113結合擬態的膜ＳＤＳ微膜 micelles□之結構，同時利用螢光光譜儀與圓二色譜來探討抗菌胜肽在膜環境的特性與穿透能力。本研究結果發現酸鹼度及不同鹽份濃度對P-113及Pac-525的效能有嚴重影响，但酸鹼度及不同鹽份濃度對D-Nal的影響有限。

Abstract I
中文摘要 II
Chapter 1 Introduction 1
Chapter 2 Anti-yeast Activities of a Novel D-β- naphthyl- alanine Substituted Antimicrobial Peptide Are Independent from Salt 4
ABSTRACT 4
INTRODUCTION 5
MATERIALS AND METHODS 6
RESULTS AND DISCUSSION 7
REFERENCES 9
Chapter 3 The pH and Salinity effects of Anti-bacterial Activity on Novel Tryptophan-Rich Peptide D-ß-naphthylalanine-Pac-525 , Pac-525 and P113 17
ABSTRACT 17
INTRODUCTION 17
MATERIALS AND METHODS 19
RESULTS 23
DISCUSSION 25
Chapter 4. Solution Structure and Model Membrane Interactions of P-113, a Clinically Active Antimicrobial Peptide Derived from Human Saliva 38
ABSTRACT 38
INTRODUCTION 39
METHOD 41
RESULTS 43
DISCUSSION 46
REFERENCES 48

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