在本研究中利用金導線陣列製作新穎之生物感測器，藉由量測肝功能重要指標-人類血清蛋白 (human serum albumin, HSA) ，期待未來整合於生物晶片中，用來即時檢測肝功能健康狀況。
在本研究中生物感測器製備方式，首先利用半導體製程製備生物感測器，其金電極間玻璃區域，以3-氨丙基三乙氧基矽烷 (3-aminopropyltriethoxysilane, APTES) 進行表面改質，使玻璃表面具有生物相容性後，依序固定上人類血清蛋白抗體 (anti-human serum albumin, AHSA) 、脫脂奶粉阻斷劑，以作為待測物人類血清蛋白 (HSA) 之接合與檢測。
在人類血清蛋白之感測與定量分析上，乃利用電化學阻抗譜 (Electrochemical Impedance Spectroscopy, EIS) ，在交流電下，直接量測接合於相鄰兩金電極間玻璃上之人類血清蛋白阻抗特性，藉由不同濃度貢獻之電容性阻抗變化量，定量分析人類血清蛋白之濃度。結果發現，當人類血清蛋白濃度越高，其貢獻的阻抗越大，且阻抗變化與人類血清蛋白濃度之對數 (log) 數值間呈線性關係；除此之外，此電化學阻抗式人類血清蛋白生物感測器，靈敏度可到達2□10-4 mg/ml。
本研究利用電化學阻抗式機制，用以定量檢測人類血清蛋白濃度，並成功證明此機制之可行性，且在感測器僅需利用單層黃光製程製作金電極陣列，並可直接製作於生物晶片上。因具備感測過程便利、易整合於生物晶片、分析結果可即時輸出、低成本等優點，對肝功能檢測上有極大應用價值。

第一章 續論 1
第二章 文獻回顧 3
2.1 生物感測器簡介 3
2.1.1 依據辨識元件 (Recognition Element) 分類生物感測器 5
2.1.2 依據傳感器 (Transducer) 分類生物感測器 8
2.2 肝功能指數量測 11
2.2.1 肝功能指數 11
2.2.2 人類血清蛋白檢測 13
第三章 實驗流程與方法 18
3.1 金陣列生物感測器製備 20
3.2 固定人類血清蛋白抗體之基板製備 22
3.2.1 3-氨基丙基三甲氧基甲矽烷 (APTES) 改質 22
3.2.2 人類血清蛋白抗體和阻斷劑固定流程 24
3.2.3 抗體和阻斷劑固定確認流程 27
3.3 接合人類血清蛋白 29
3.3.1 接合人類血清蛋白流程 29
3.3.2 人類血清蛋白接合之確認流程 29
3.4 運用電化學阻抗譜做人類血清蛋白之電性量測 31
3.5 儀器簡介 34
3.5.1 原子力電子顯微鏡 (Atomic Force Microscopy) 34
3.5.2 掃描式電子顯微鏡 (Scanning Electron Microscopy) 36
3.5.3 螢光顯微鏡 (Fluorescence Microscopy) 37
3.5.4 電性量測儀器 39
第四章 實驗結果與討論 40
4.1 利用掃描式和原子力顯微鏡觀察生物感測表面改質形貌 40
4.2 螢光抗體確認生物感測器表面改質 43
4.2.1 人類血清蛋白抗體固定和脫脂奶粉阻斷固定之確認 43
4.2.2 人類血清蛋白接合之確認 46
4.3 電性量測分析 47
4.3.1 等效電路分析 47
4.3.2 電性量測機制最佳化 51
4.3.3 電化學阻抗量測技術最佳化 54
4.3.4 量測頻率範圍分析 58
4.3.5 起始阻抗值 (ZAHSA+Blocking) 條件分析 60
4.3.6 人類血清蛋白在金電極通道間之阻抗特性 62
4.4 人類血清蛋白濃度定量分析 67
第五章 結論 74
第六章 未來展望 77
第七章 參考文獻 79
本研究產出之論文發表 84

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