為了提升奈米碳管在熱治療上的效率及未來實際應用的可能性，本研究嘗試將多壁奈米碳管與抗體相結合，並以近紅外光照射加熱，觀察細胞的生存狀況。以本實驗室自產的多壁奈米碳管，經表面改質處理，成功的與anti-HER2 antibody相結合。在進行後續實驗前以螢光免疫染色法進行確認。
照光實驗以Alamar Blue細胞生存率測試作定量統計。結果顯示，帶有抗體的多壁奈米碳管無論是在殺死乳癌細胞的能力或細胞的選擇性，都優於官能基化碳管。此外添加碳管濃度的不同、照光時間差異或是照光結束後於不同時間點下進行統計，都會影響到此實驗結果。
以EtBr螢光染劑對照光完的細胞進行染色，其結果與定量統計實驗結果相呼應。亦藉著EtBr染色機制，確認了細胞膜在照光實驗結束後，有損毀的情形，這導致了細胞的死亡。
本研究進一步以共軛聚焦顯微鏡證明，帶有抗體的碳管與乳癌細胞相接觸後能藉由胞吞作用進入細胞。一方面可解釋此實驗組具有較高殺死癌細胞的效果，也證明了本研究殺死癌細胞的機制可能存在內部蛋白質結構破壞的原因。

第1章 緒論1
1-1 奈米碳管的簡介 1
1-1-1 奈米碳管的起源與結構 1
1-1-2 奈米碳管的製備 3
1-2 細胞的簡介 5
1-2-1 細胞的定義與分類 5
1-2-2 細胞的組成與結構 6
1-2-3 細胞的生長需求 7
1-2-4 人類表皮生長因子受體2蛋白質(Human epidermal growth factor receptor 2 protein, HER2) 7
1-2-5 單株抗體 8
1-2-6 免疫化學染色法(IHC) 8
1-3 奈米碳管與生物科學領域 9
1-3-1 奈米碳管在生醫工程上的應用 9
1-3-2 奈米碳管對細胞的影響與生物的影響 11
1-3-3 奈米碳管結合光照在生醫領域上的應用 13
1-4 研究動機 14
第2章 帶有抗體的多壁奈米碳管溶液製備 29
2-1 多壁奈米碳管(MWCNTs)的製備 29
2-1-1 多壁奈米碳管粉末的製備 29
2-1-2 官能基化(Functionalization)多壁奈米碳管之製備 30
2-2 原始與官能基化多壁奈米碳管形貌與性質分析 31
2-3 帶有抗體的多壁奈米碳管溶液之製備 32
2-4 以二抗帶螢光(anti-rat IgG-dylight 488)染劑確認奈米碳管與anti-HER2 antibody結合情形 35
2-5 帶螢光、抗體的多壁奈米碳管與細胞接觸的情形 36
第3章 以奈米碳管作吸收近紅外光媒介對癌症細胞造成的影響 43
3-1 官能基化多壁奈米碳管溶液以808 nm近紅外光照射後升溫現象 43
3-2 帶抗體的多壁奈米碳管以近紅外線照射後對於乳癌細胞影響的定量統計 44
3-2-1染色時間點在奈米碳管照射紅外線殺死乳癌細胞實驗對細胞的影響 44
3-2-2 抗體銜接、碳管濃度在奈米碳管照射紅外線殺死乳癌細胞實驗中的影響 47
3-2-3 不同照光時間對殺死癌細胞能力的影響 48
3-2-4 以第二種癌細胞-大腸癌細胞HCT-8進行奈米碳管照光實驗 48
3-3 以EtBr螢光染劑對奈米碳管光照實驗的結果作分析比較 49
第4章 結果與討論 54
4-1 原始與官能基化多壁奈米碳管形貌與性質分析結果 54
4-1-1 場發掃描式電子顯微鏡觀察材料之結果 54
4-1-2 拉曼光譜儀之分析結果 54
4-1-3傅利葉轉換紅外線光譜儀觀察多壁奈米碳管官能基前後之差異 56
4-2 以anti-rat IgG-dylight 488確認奈米碳管與anti-HER2 antibody結合結果 57
4-3 帶螢光及抗體的多壁奈米碳管與細胞接觸時的現象 59
4-4 官能基化多壁奈米碳管水溶液以808 nm近紅外光光源照射後升溫現象結果 60
4-5 帶抗體的多壁奈米碳管以近紅外線照射後對於乳癌細胞的影響 60
4-5-1 染色時間點對於定量結果的影響之結果 60
4-5-2 抗體銜接與碳管濃度的效應 61
4-5-3 不同照光時間對癌細胞生存力的影響 63
4-5-4 HCT-8大腸癌細胞進行奈米碳管照光實驗 64
4-6以螢光染劑對奈米光照實驗的結果作分析比較之結果 65
第5章 結論 94
參考文獻 97

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