近年來，奈米碳管被廣泛應用在各個領域。其存在的潛在危險是奈米碳管可能在製造、使用或廢棄的過程中，逸散到生活環境當中，危害生物體。為了探究此可能性，諸多學者開始探討奈米碳管對各種植物生長的影響及可能的機制。然而，卻鮮少有研究學者討論植物攝取奈米碳管的可能性以及奈米碳管進入的運輸途徑等相關之課題。故本研究首先觀察奈米碳管對綠豆生長過程所造成的影響，並嘗試對奈米碳管進入植物中的運輸途徑進行一探討，最後進一步思考思考奈米碳管影響植物生長的可能機制
本實驗，分別以光學顯微鏡、環境掃描式電子顯微鏡、場發射掃描式電子顯微鏡觀察比較有無奈米碳管共同培養的植物形貌之差異，並由數據統計發現奈米碳管具有明顯促進綠豆芽伸長的能力，尤其在根部的長度最為明顯，比控制組平均增長了10-13 mm，而莖部則是平均增長5-8 mm。但是，與少壁奈米碳管溶液共同培養的綠豆細胞形貌有破損的現象產生。本研究進一步運用熱差分析儀偵測不同培養條件的綠豆芽蘊含水含量多寡，結果顯示，奈米碳管可能促進綠豆水分的吸收。另一方面，本研究亦使用了拉曼光譜儀以及場發射掃描電子顯微鏡探討與證實奈米碳管是否確實被攝取進入植物體內。
最後，本研究利用非侵入式3D活體影像系統追蹤已接上螢光的奈米碳管進入植物及在植物體內運輸的途徑，並利用共軛聚焦顯微鏡進行更細部的微觀分析與觀測，結果顯示帶螢光的少壁奈米碳管主要存在於細胞壁之間。由此推論，其可能的運輸途徑為經由質體外運輸進入綠豆植株。

第1章 緒論..............................................1
1-1 奈米碳管的簡介......................................1
1-1-1奈米碳管的起源與結構...............................1
1-1-2 奈米碳管的製備....................................3
1-2 植物的簡介..........................................5
1-2-1 植物的演化和分類..................................5
1-2-2 植物的構造........................................7
1-2-3 植物的運輸作用....................................8
1-3奈米碳管與生物科學領域...............................9
1-3-1 奈米碳管在生醫工程的應用..........................9
1-3-2 奈米碳管對細胞的影響.............................10
1-3-3奈米碳管對植物的影響..............................12
1-4 研究動機...........................................14
第2章 帶螢光的少壁奈米碳管溶液製備.....................30
2-1官能基化(Functionalized)少壁奈米碳管之製備..........30
2-2 原始、官能基化少壁奈米碳管之形貌與性質分析.........31
2-3 帶螢光少壁奈米碳管溶液(Dy-LWCNTs medium)之製備.....32
2-4 利用螢光顯微鏡觀察帶螢光少壁奈米碳管之結合情形.....34
第3章 分析與少壁奈米碳管共同培養的綠豆植株之實驗方法...40
3-1 綠豆種子（Mung Bean）的滅菌與植株的培養............40
3-2　分析與少壁奈米碳管共同培養的綠豆植株之生長統計與形貌觀察的實驗方法...........................................41
3-3 利用熱重分析儀測量綠豆植株中水分含量多寡之實驗方法.45
3-4 利用拉曼光譜儀偵測綠豆植株中少壁奈米碳管之實驗方法.45
3-5 利用非侵入式3D活體影像分子系統(In Vivo Image System)追蹤帶螢光少壁奈米碳管之實驗方法...........................41
3-6 利用共軛聚焦顯微鏡(Confocal Microscopy)追蹤帶螢光少壁奈米碳管之實驗方法.......................................47
第4章 結果與討論.......................................53
4-1 原始與官能基化少壁奈米碳管形貌與性質分析結果.......53
4-1-1 傅利葉轉換紅外線光譜儀觀察少壁奈米碳管之結果.....53
4-1-2 拉曼光譜儀觀察少壁奈米碳管之結果.................54
4-1-3 場發掃描式電子顯微鏡及穿透式電子顯微鏡觀察少壁奈米碳管之結果.................................................55
4-1-4 高解析電子能譜儀觀察少壁奈米碳管之結果...........56
4-1-5 熱重分析儀觀察少壁奈米碳管之結果.................57
4-2 帶螢光的少壁奈米碳管形貌與性質分析結果.............58
4-3 少壁奈米碳管對綠豆生長之影響.......................59
4-4 少壁奈米碳管對綠豆芽含水量的影響...................61
4-5 少壁奈米碳管在綠豆植株中存在位置的探討.............63
4-6 少壁奈米碳管在綠豆的運輸途徑之探討.................65
第5章 結論.............................................99
參考文獻..............................................102

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