金屬離子與誘引劑處理對丹參毛狀根生長與丹參酮累積之影響

Influence of Metal Ion and Elicitor on Hairy Root Growth and Production of Tanshinones of Salvia miltiorrhiza

陳威臣(Uei-Chern Chen) ； 李秋儀(Choi-Yi Lee) ； 詹効松(Hsiao-Sung Chan) ； 曹進義(Chin-Yi Tsao) ； 夏奇鈮(Chi-Ni Hsia)

《台灣農業研究》 59卷1期 (2010/03) Pp. 49-60

https://doi.org/10.6156/JTAR/2010.05901.05

摘要

本研究以丹參(Salvia miltiorrhiza Bunge)毛狀根為材料，測試在液態培養中添加金屬離子與誘引劑(elicitors)對毛狀根生長與丹參酮(tanshinones)累積之影響。添加銅、鐵、錳及鈷金屬離子之試驗結果顯示，除鈷離子對毛狀根乾重未達顯著促進效果外，鐵、錳及銅離子的添加皆有利於毛狀根乾重增加；適當濃度之鐵、錳及鈷離子可促進毛狀根之丹參酮累積，其中以100 μM鐵離子處理效果最佳，丹參酮總產量可達對照組之1.8倍。誘引劑處理顯示添加酵母誘引劑、茉莉酸及離層酸對毛狀根生長無不良影響，且除水楊酸處理外，皆可促進丹參酮含量的累積，其中以2 mg/L離層酸處理之丹參酮含量為對照組的3.4倍效果最佳。進一步測試以不同濃度離層酸配合處理時間的結果顯示，三種濃度(1、2、4 mg/L)離層酸處理均可促進丹參酮含量累積，其中以1 mg/L離層酸處理3週後之效果最佳，丹參酮累積達22.9 mg/L，促進效果主要在於隱丹參酮與丹參酮IIA含量的增加。

關鍵字

丹參 ； 金屬離子 ； 誘引劑 ； 離層酸 ； 酵母誘引劑 ； 二次代謝物 ； 丹參酮

並列摘要

The objective of this study was to examine the effect of metal ions and elicitors on root growth and tanshinones production of Salvia miltiorrhiza hairy root culture. Metal ions of Cu(superscript 2+), Fe(superscript 2+), Mn(superscript 2+), and Co(superscript 2+) with various concentrations were tested. Results showed except Co(superscript 2+) treatment that Fe(superscript 2+), Mn(superscript 2+), and Cu(superscript 2+) were all able to increase the dry weight of hairy roots, significantly comparing the control treatment. Moreover, applications of suitable concentration of Fe(superscript 2+), Mn(superscript 2+) and Co(superscript 2+) into the medium could promote tanshinones accumulation. Among them, the 100 μM Fe(superscript 2+) treatment the highest yield of tanshinones had a 1.8-fold increase than that of the control. Elicitors of yeast elicitor, jasmonic acid, abscisic acid and salicylic acid were found no effect on root growth however only the first three could enhance tanshinones production. Among elicitor treatments, best result with a 3.4-fold increase on tanshinones production was found by using 2 mg/L ABA than the control. Consecutive experiment using 1, 2, and 4 mg/L ABA in combination with expose duration was conducted. All ABA treatments resulted in higher tanshinones production than that of the control. The highest tanshinones production 22.9 mg/L was found using 1 mg/L ABA for 3-weeks exposure duration and cryptotanshinone and tanshinone IIA were counted for the major increasing part of the yield.

並列關鍵字

Danshen ； Salvia miltiorrhiza ； Metal ion ； Elicitator ； Abscisic acid ABA ； Yeast elicitor YE ； Secondary metabolites ； Tanshinones ； Hairy root culture