- 期刊
- OpenAccess
Plasmodium falciparum Pfs25 Gene Promoter Has no Polymorphism in Natural Isolates of Eastern India
並列摘要
Most of the potential vaccine candidate genes show polymorphism or point mutation, which affects the structure of antigen and thereby, production of antibody to protect the host against further infection. In the present study, we have analyzed the promoter regions of 415 base pair (nucleotide position -722 to -308) of Pfs25 gene. Previous studies have shown that, removing these important regions drastically affects the promoter activity and Pfs25 protein expression. We for the first time, report that though promoter regions of Pfs25 gene is AT rich, it doesn’t show any polymorphism or point mutation in sequences important for its activity in 155 natural isolates of Plasmodium falciparum from hyper endemic malaria transmission regions. Since, Pfs25 expression is vital for the survival of the parasite in the mosquito vector, a stable promoter sequence of Pfs25 in all the strains may have fair chances of survival in the mosquito vector. Our finding of a stable promoter sequence in combination with previous reports of limited polymorphism in the coding regions of Pfs25 indicates that Pfs25 might be a strong candidate for vaccine.
延伸閱讀
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