- 期刊
- OpenAccess
Molecular Cloning and Functional Characterization of Protein Phosphatase 2C of Two Scuticociliates-Uronema marinum and Miamiensis avidus (Ciliophora: Scuticociliatia)
並列摘要
Complementary DNAs (cDNAs) of protein phosphatase 2C (PP2C) were cloned from two marine scuticociliates Uronema marinum and Miamiensis avidus. Both PP2C proteins showed structural characteristics of typical PP2C, such as highly conserved amino acid residues predicted for binding to phosphate and metal ions, 11 conserved PP2C motifs and 10 invariant residues. The phosphatase activity of recombinantly produced U. marinum PP2C (UmPP2C) was in proportion to the PP2C protein and Mg(superscript 2+) concentrations, and was not sensitive to okadaic acid, but was inhibited by sodium fluoride, EDTA or Ca(superscript 2+). The expression of UmPP2C was significantly up-regulated by exposure the ciliates with PMA suggesting that UmPP2C dephosphrylates proteins phosphorylated by protein kinases as in other eukaryotes and has a regulatory function against abrupt increase of protein phosphorylation triggered by strong stimulations.
延伸閱讀
- 林培茵（2011）。Molecular cloning and expression of the cellulase gene from halophiles Bacillus licheniformis NTU-01〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2011.00121
- Mushir, S., & Fatma, T. (2011). Ultraviolet Radiation-absorbing Mycosporine-like Amino Acids in Cyanobacterium Aulosira fertilissima: Environmental Perspective and Characterization. Current Research Journal of Biological Sciences, 3(2), 165-171. https://www.airitilibrary.com/Article/Detail?DocID=20410778-201103-201512240016-201512240016-165-171
- 張興慈（2010）。Molecular Cloning and Characterization of an Insecticidal Protein from Pseudomonas taiwanensis TKU015〔碩士論文，淡江大學〕。華藝線上圖書館。https://doi.org/10.6846/TKU.2010.00097
- Shang, H. M., Song, W. B., & Warren, A. (2003). Phylogenetic Positions of Two Ciliates, Paranophrys magna and Mesanophrys carcini (Ciliophora: Oligohymenophorea), within the Subclass Scuticociliatia Inferred from Complete Small Subunit rRNA Gene Sequences. Acta Protozoologica, 42(3), 171-181. https://www.airitilibrary.com/Article/Detail?DocID=00651583-200309-201012290010-201012290010-171-181
- PAN, X. M., Huang, J., Fan, X. P., Ma, H. G., RASHEID, K. A. S. A., MIAO, M., & Gao, F. (2015). Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.. Acta Protozoologica, 54(1), 31-43. https://doi.org/10.4467/16890027AP.15.003.2190