摘要
本研究之目的在嘗試開發出一個簡單和快速檢測飼料中clenbuterol之方法。先以市售商業化檢測β-agonist之酵素檢驗套組進行樣品篩選工作，擬陽性樣品再以高效液相層析法搭配紫外光檢測器及電化學檢測器進行檢測。結果顯示樣品僅須經簡單地抽提後，通過Extrelut20管柱即可進行分析。利用Cosmosil 5C18-MS分離管，以25%氰甲烷溶液加0.01M三水醋酸納緩衝液（利用醋酸調pH至3.5）為移動相，於紫外光檢測波長244nm和電化學檢測+1250mV下進行分析，可成功地自飼料中檢測出clenbuterol，並且平均回收率可達79.4%。
並列摘要
The aim of this study is to develop a simple, rapid, and reliable method for determination of clenbuterol in feeds. Samples were performed by using enzyme-linked immunosorbent assay (ELISA) as a screening method. Positive samples were confirmed by high performance liquid chromatography (HPLC) with series of ultraviolet and electrochemical detection. Clenbuterol in the sample was extracted with water (pH 3.5), followed by an Extrelut 20 column clean-up and HPLC as the means of measurement. Analysis was carried out on a Cosmosil 5C18-MS column (Nacalai, Japan) with acetonitrile-0.01 M sodium acetate buffer (25:75 v/v, pH 3.5 adjusted with acetic acid ) as the mobile phase, and was detected both by UV detector (at 244nm) and electrochemical detector (at + 1250 mV). The results revealed that clenbuterol in feeds was detected in 7.9 min, and the average recovery was 79.4%.
被引用紀錄
延伸閱讀
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