The middle internodes of each bamboo culm height were cut from Brachystachyum densiflorum, Bashania fargesii, Bambusa multiplex var. nana and Chimonobambusa pachystachys which were collected from the Zhejiang Province, Mainland China. The investigation of anatomical structure of those four bamboo species were used not only sectional method but also light microscope and scanning electron microscope (SEM). The structural investigations of those two bamboo species were concentrated on cuticular layer, epidermis, subepidermis cortex, vascular bundle, fundamental parenchyma, pith peri-pheral parenchyma, bamboo membrane, cell inclusion, crystal of calcium and silica and multilayer structure of cell wall. The detail data of structure were summarized in some tables which can be comparative with each other.