台灣杉心材抗菌成分之研究（Ⅰ）正己烷可溶部抗菌成分之分離與鑑定

Studies on the Antifungal Compounds in the Heartwood Extractives of Taiwania (Taiwania cryptomerioides Hayata) (I): Isolation and Identification of Antifungal Compounds in Hexane Soluble Fraction

張上鎮(Shang-Tzen Chang) ； 吳季玲(Chi-Lin Wu) ； 王升陽(Sheng-Yang Wang) ； 蘇裕昌(Yu-Chang Su) ； 郭悅雄(Yueh-Hsiung Kuo)

《林產工業》 17卷2期 (1998/06) Pp. 287-303

摘要

本研究係探討台灣杉心材抽出物與其抗菌性質之相關性，由台灣杉心材甲醇抽出物之正己烷可溶部所分離出之5種化合物，經質譜、核磁共振氫譜、核磁共振碳譜及二維核磁共振光譜等分析技術鑑定，確定此5種化合物分別為Taiwanin A、α-Cadinol、a-Cedrol、Hinokiol和Sugiol。由抗菌性試驗得知上述5種化合物在100 ppm的濃度下，對白腐菌（Coriolus versicolor (L. ex Fr. ) Quel. ）之抗菌指數大小順序分別為：α-Cadinol＞α-Cedrol＞Hinokiol＞Sugiol＞Taiwanin A；對褐腐菌（Laetiporus sulphureus (B. ex Fr. ）Bond. ）則為：α-Cadinol＞Hinokiol＞α-Cedrol＞Taiwanin A。其中，α-Cadinol在100 ppm濃度時幾乎可完全抑制白腐菌（C. versicolor）及褐腐菌（L. sulphureus）之生長，顯示出極強之抗菌性。

關鍵字

臺灣杉 ； 心材 ； 抗菌成分 ； 白腐菌 ； 褐腐菌 ； 杜松子油醇

並列摘要

This study identified the compounds in the heartwood extractives of Taiwania (Taiwania cryptomerioides Hayata) which inhibited the mycelial growth of white-rot fungus (Coriolus versicolor (L. ex Fr. ) Quel. ) and brown-rot fungus (Laetiporus sulphureus (B. ex Fr. ) Bond. ). Five compounds were isolated from hexane solution fraction of the methanol extractives of Taiwania heartwood. According to Mass, ^1H-NMR, ^(13)C-NMR, and 2D-NMR analysis, their structures were identified, namely Taiwanin A, α-Cadinol,α-Cedrol, Hinokiol, and Sugiol. Based on the results of antifungal test, the order of antifungal index of these compounds for C. versicolor was α-Cadinol＞α-Cedrol＞Hinokiol＞Sugiol＞Taiwanin A. For L. sulphureus, it was α-Cadinol＞Hinokiol＞α-Cedrol＞Taiwanin A. Among these, α-Cadinol showed the best antifungal effectiveness. It completely inhibited the growth of C. versicolor and L. sulphureus at 100 ppm.

並列關鍵字

Taiwania (Taiwania cryptomerioides Hayata) ； Heartwood ； Antifungal compounds ； White-rot fungus ； Brown-rot fungus ； α-Cadinol