The Inhibitory Effect of Anticonvulsants of Human Sperm Motility: Measured with a Trans-Membrane Migration Method

以過膜游動法測量抗癲癇藥物對人類精蟲活動力的影響

沈孟儒(Meng-Ru Shen) ； 陳順勝(Shun-Sheng Chen)

《The Kaohsiung Journal of Medical Sciences》 6卷6期 (1990/06) Pp. 295-301

https://doi.org/10.6452/KJMS.199006.0295

摘要

越來越多的證據顯示抗癲癇藥物的男性患者會減低性慾及增加形態不正常的精蟲比例。本研究以過膜游動法來測量四種抗癲癇藥物（carbamazepine，phenytoin，phenobarbitone，valproate）在試管中對人類精蟲活動力的作用。實驗分成A、B兩組進行：A組精液與藥物混合後，馬上測定其精蟲活動力；B組是將精液與藥物的混合液有37℃的昷度中先培養兩小時，再測量其精蟲活動力。實驗結果顯示，在B組中，phenytoin，carbamazepine，valproate三種藥物能減低精蟲活動力至控制組精蟲活動力的50%所需之濃度（EC50）分別為1.59，4.23，5.00 mM。但在A組中carbamazepine和valproate二種藥物的EC50將增加至8.47和10.00 mM，而phenytoin則不能減少精蟲活動力低於控制組精蟲活動力的50%。對phenobarbitone而言，事先培養與否，均不能降低精蟲活動力少於控制組精蟲活動力的50%。本研究再度證明具有膜穩定作用的藥物抑制精蟲活動力的推論，並且肯定人類精蟲可以應用於藥物研究的細胞模式。

並列摘要

The in-vitro effects of four anticonvulsant drugs (phenytoin, phenobarbitone, carbamazepine and valproate) on human sperm motility were studied with a trans-membrane migration method. Sperm motility was measured either immediately after semen had been mixed with the drug or after a 2-hour pre-incubation at 37℃. When the drug effect was evaluated after the semen-drug mixture had been pre-incubated for 2 hours, the concentration of phenytoin, carbamazepine and valproate that inhibited sperm motility to 50% of control (EC50) was 1.59, 4.23, and 5.00 mM, respectively. If sperm motility was immediately measured without preincubation, the EC50 was increased to 8.47 and 100.00 mM for carbamazepine and valproate, respectively, whereas phenytoin could not inhibit sperm motility to less than 50% of the control at the tested concentrations. Both with and without pre-incubation, phenobarbitone, even up to 12.92 mM, could not inhibit sperm motility to less than 50% of the control. The result was compatible with previous findings drugs with a membrane stabilizing effect may inhibit human sperm motility. This study provided further information regarding the application of human sperm motility as a cellular for future pharmacological research.

並列關鍵字

sperm motility ； anticonvulsant ； membrane stabilizing effect